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Cellosaurus LN-464 (CVCL_3962)

[Text version]

Cell line name LN-464
Synonyms LN 464; LN464
Accession CVCL_3962
Resource Identification Initiative To cite this cell line use: LN-464 (RRID:CVCL_3962)
Comments Problematic cell line: Probably contaminated. The STR profile of ACN and LN-464 are identical. SNP analysis (DepMap) also shows identity of both cell lines. We believe this is a melanoma cell line based on the presence of the BRAF V600E mutation as well as the RNA-Seq expression profile of LN-464 (DepMap) which clusters with that of melanoma patient samples. Originally thought to originate from the Brain; right parieto-occipital lobe of a 67 year old male patitne with a glioblastoma.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Doubling time: 35 hours (PubMed=25984343).
Omics: Deep exome analysis.
Omics: shRNA library screening.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (DepMap).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Asn184fs*6 (c.545_546insA); Zygosity=Heterozygous (PubMed=10416987; DepMap).
  • Mutation; HGNC; 11998; TP53; Simple; p.Phe113Ser (c.338T>C); ClinVar=VCV000823709; Zygosity=Homozygous (PubMed=10416987; DepMap).
Disease Melanoma (NCIt: C3224)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_1068 ! ACN
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): PubMed=22570425

Markers:
AmelogeninX
CSF1PO11
D3S135816
D5S81812
D7S82010,13
D8S117912,13
D13S31711,13
D16S53911
D18S5116
D21S1128
FGA22
Penta D9
Penta E10,14
TH019
TPOX9,12
vWA14,17,18

Run an STR similarity search on this cell line
Publications

PubMed=7693337
Li H., Hamou M.-F., de Tribolet N., Jaufeerally R., Hofmann M., Diserens A.-C., Van Meir E.G.
Variant CD44 adhesion molecules are expressed in human brain metastases but not in glioblastomas.
Cancer Res. 53:5345-5349(1993)

PubMed=8509230; DOI=10.1002/ijc.2910540329
Rimoldi D., Romero P., Carrel S.
The human melanoma antigen-encoding gene, MAGE-1, is expressed by other tumour cells of neuroectodermal origin such as glioblastomas and neuroblastomas.
Int. J. Cancer 54:527-528(1993)

PubMed=10416987; DOI=10.1111/j.1750-3639.1999.tb00536.x
Ishii N., Maier D., Merlo A., Tada M., Sawamura Y., Diserens A.-C., Van Meir E.G.
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Brain Pathol. 9:469-479(1999)

PubMed=22570425; DOI=10.1093/neuonc/nos072
Bady P., Diserens A.-C., Castella V., Kalt S., Heinimann K., Hamou M.-F., Delorenzi M., Hegi M.E.
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line databases/resources Cell_Model_Passport; SIDM00058
DepMap; ACH-000676
Anatomy/cell type resources BTO; BTO:0004451
Biological sample resources BioSample; SAMN10988403
Chemistry resources PharmacoDB; LN464_848_2019
Encyclopedic resources Wikidata; Q54902786
Polymorphism and mutation databases Cosmic; 849873
Cosmic; 2367498
Cosmic; 2516032
Entry history
Entry creation04-Apr-2012
Last entry update23-Jun-2022
Version number19