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Cellosaurus RBL-2H3 (CVCL_0591)

[Text version]
Cell line name RBL-2H3
Synonyms RBL2H3; RBL 2H3; RBL.2H3
Accession CVCL_0591
Resource Identification Initiative To cite this cell line use: RBL-2H3 (RRID:CVCL_0591)
Comments Characteristics: Degranulates as a consequence of Fc-epsilon-R stimulation. Histamine-releasing (PubMed=19669619).
Characteristics: Compound 48/80 which is used to promote mast cell degranulation is toxic to this cell line (DOI=10.57844/arcadia-3207-4695).
Doubling time: ~50-60 hours (DSMZ=ACC-312).
Transformant: ChEBI; CHEBI_232042; Beta-chloro-N-isopropyl-2-naphthaleneethanamine hydrochloride (ICI 42464).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: Basophil; CL=CL_0000767.
Disease Rat leukemia (NCIt: C60428)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Wistar; RS=RS_0000955.
Hierarchy Parent: CVCL_E3P7 (RBL-IV HR+)
Children:
CVCL_C4MP (RBL SX-38)CVCL_KU64 (RBL-2H3 ADORA2A Gq)CVCL_KU65 (RBL-2H3 MRGPRX1 Gq)
CVCL_C4MQ (RBL-30/25)CVCL_C4MR (RBL-hEIalpha-2B12)CVCL_1D84 (TB1A2)
Category Cancer cell line
Web pages Info; protocols.io; -; https://dx.doi.org/10.17504/protocols.io.14egn67qpl5d/v1
Publications

PubMed=6166481; DOI=10.1002/eji.1830110410
Edward L. Barsumian, Chaviva Isersky, Marianne G. Petrino, Reuben P. Siraganian;
IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones.
Eur. J. Immunol. 11:317-323(1981)

PubMed=6173265
Reuben P. Siraganian, Anne McGivney, Edward L. Barsumian, Fulton T. Crews, Fusao Hirata, Julius Axelrod;
Variants of the rat basophilic leukemia cell line for the study of histamine release.
Fed. Proc. 41:30-34(1982)

PubMed=2940298; DOI=10.4049/jimmunol.136.12.4652
Toshiomi Okuno, Yutaka Takagaki, Dov H. Pluznik, Julie Yin Djeu;
Natural cytotoxic (NC) cell activity in basophilic cells: release of NC-specific cytotoxic factor by IgE receptor triggering.
J. Immunol. 136:4652-4658(1986)

PubMed=3262677; DOI=10.4049/jimmunol.141.9.3061
Allen L. Richards, Toshiomi Okuno, Yutaka Takagaki, Julie Yin Djeu;
Natural cytotoxic cell-specific cytotoxic factor produced by IL-3-dependent basophilic/mast cells. Relationship to TNF.
J. Immunol. 141:3061-3066(1988)

PubMed=17210052; DOI=10.1111/j.1365-2222.2006.02618.x
Susanne Kaul, Dirk Luttkopf, Birgit Kastner, Lothar Vogel, Gerhard Holtz, Stefan Vieths, Andrea Hoffmann;
Mediator release assays based on human or murine immunoglobulin E in allergen standardization.
Clin. Exp. Allergy 37:141-150(2007)

PubMed=19301096; DOI=10.1007/s00011-009-0028-4
Egle Passante, Carsten Ehrhardt, Helen Sheridan, Neil Frankish;
RBL-2H3 cells are an imprecise model for mast cell mediator release.
Inflamm. Res. 58:611-618(2009)

PubMed=19669619; DOI=10.1007/s00011-009-0074-y
Egle Passante, Neil Frankish;
The RBL-2H3 cell line: its provenance and suitability as a model for the mast cell.
Inflamm. Res. 58:737-745(2009)

PubMed=25149487; DOI=10.1007/978-1-4939-1173-8_8
Egle Passante;
Mast cell and basophil cell lines: a compendium.
Methods Mol. Biol. 1192:101-113(2014)

PubMed=36125550; DOI=10.1007/s00441-022-03687-w
Chuda Rujitharanawong, Sunisa Yoodee, Kanyarat Sueksakit, Paleerath Peerapen, Papapit Tuchinda, Kanokvalai Kulthanan, Visith Thongboonkerd;
Systematic comparisons of various markers for mast cell activation in RBL-2H3 cells.
Cell Tissue Res. 390:413-428(2022)

DOI=10.57844/arcadia-3207-4695
MaryClare Rollins, Edgard Morazan, Adair L. Borges, Justin Donnelly;
Compound 48/80 is toxic in HMC1.2 and RBL-2H3 cells.
Arcadia 2025:0317.1-0317.18(2025)

Cross-references
Cell line collections (Providers) AddexBio; C0003007/376
ATCC; CRL-2256
BCRC; 60310
CCTCC; GDC0213
CLS; 305194
DSMZ; ACC-312
JCRB; JCRB0023
KCLB; 22256
NCBI_Iran; C536
RCB; RCB2782
TKG; TKG 0321
Cell line databases/resources CLO; CLO_0008763
CLO; CLO_0051024
MCCL; MCC:0000394
CCRID; 1101RAT-PUMC000817
CCRID; 3101RATSCSP518
CCRID; 3101RATTCR7
CCRID; 4201SWI-CCTCC00213
DSMZCellDive; ACC-312
Lonza; 1035
Anatomy/cell type resources BTO; BTO_0000833
Chemistry resources ChEMBL-Cells; CHEMBL3307666
ChEMBL-Targets; CHEMBL614632
PubChem_Cell_line; CVCL_0591
Encyclopedic resources Wikidata; Q54949346
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number27