ID   RBL-2H3
AC   CVCL_0591
SY   RBL2H3; RBL 2H3; RBL.2H3
DR   BTO; BTO_0000833
DR   CLO; CLO_0008763
DR   CLO; CLO_0051024
DR   MCCL; MCC:0000394
DR   AddexBio; C0003007/376
DR   ATCC; CRL-2256
DR   BCRC; 60310
DR   CCRID; 1101RAT-PUMC000817
DR   CCRID; 3101RATSCSP518
DR   CCRID; 3101RATTCR7
DR   CCRID; 4201SWI-CCTCC00213
DR   CCTCC; GDC0213
DR   ChEMBL-Cells; CHEMBL3307666
DR   ChEMBL-Targets; CHEMBL614632
DR   CLS; 305194
DR   DSMZ; ACC-312
DR   DSMZCellDive; ACC-312
DR   JCRB; JCRB0023
DR   KCLB; 22256
DR   Lonza; 1035
DR   NCBI_Iran; C536
DR   PubChem_Cell_line; CVCL_0591
DR   RCB; RCB2782
DR   TKG; TKG 0321
DR   Wikidata; Q54949346
RX   DOI=10.57844/arcadia-3207-4695;
RX   PubMed=2940298;
RX   PubMed=3262677;
RX   PubMed=6166481;
RX   PubMed=6173265;
RX   PubMed=17210052;
RX   PubMed=19301096;
RX   PubMed=19669619;
RX   PubMed=25149487;
RX   PubMed=36125550;
WW   Info; protocols.io; -; https://dx.doi.org/10.17504/protocols.io.14egn67qpl5d/v1
CC   Characteristics: Degranulates as a consequence of Fc-epsilon-R stimulation. Histamine-releasing (PubMed=19669619).
CC   Characteristics: Compound 48/80 which is used to promote mast cell degranulation is toxic to this cell line (DOI=10.57844/arcadia-3207-4695).
CC   Doubling time: ~50-60 hours (DSMZ=ACC-312).
CC   Transformant: ChEBI; CHEBI_232042; Beta-chloro-N-isopropyl-2-naphthaleneethanamine hydrochloride (ICI 42464).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
CC   Cell type: Basophil; CL=CL_0000767.
CC   Breed/subspecies: Wistar; RS=RS_0000955.
DI   NCIt; C60428; Rat leukemia
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_E3P7 ! RBL-IV HR+
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 27
//
RX   DOI=10.57844/arcadia-3207-4695;
RA   Rollins M., Morazan E., Borges A.L., Donnelly J.;
RT   "Compound 48/80 is toxic in HMC1.2 and RBL-2H3 cells.";
RL   Arcadia 2025:0317.1-0317.18(2025).
//
RX   PubMed=2940298; DOI=10.4049/jimmunol.136.12.4652;
RA   Okuno T., Takagaki Y., Pluznik D.H., Djeu J.Y.;
RT   "Natural cytotoxic (NC) cell activity in basophilic cells: release of
RT   NC-specific cytotoxic factor by IgE receptor triggering.";
RL   J. Immunol. 136:4652-4658(1986).
//
RX   PubMed=3262677; DOI=10.4049/jimmunol.141.9.3061;
RA   Richards A.L., Okuno T., Takagaki Y., Djeu J.Y.;
RT   "Natural cytotoxic cell-specific cytotoxic factor produced by
RT   IL-3-dependent basophilic/mast cells. Relationship to TNF.";
RL   J. Immunol. 141:3061-3066(1988).
//
RX   PubMed=6166481; DOI=10.1002/eji.1830110410;
RA   Barsumian E.L., Isersky C., Petrino M.G., Siraganian R.P.;
RT   "IgE-induced histamine release from rat basophilic leukemia cell
RT   lines: isolation of releasing and nonreleasing clones.";
RL   Eur. J. Immunol. 11:317-323(1981).
//
RX   PubMed=6173265;
RA   Siraganian R.P., McGivney A., Barsumian E.L., Crews F.T., Hirata F.,
RA   Axelrod J.;
RT   "Variants of the rat basophilic leukemia cell line for the study of
RT   histamine release.";
RL   Fed. Proc. 41:30-34(1982).
//
RX   PubMed=17210052; DOI=10.1111/j.1365-2222.2006.02618.x;
RA   Kaul S., Luttkopf D., Kastner B., Vogel L., Holtz G., Vieths S.,
RA   Hoffmann A.;
RT   "Mediator release assays based on human or murine immunoglobulin E in
RT   allergen standardization.";
RL   Clin. Exp. Allergy 37:141-150(2007).
//
RX   PubMed=19301096; DOI=10.1007/s00011-009-0028-4;
RA   Passante E., Ehrhardt C., Sheridan H., Frankish N.;
RT   "RBL-2H3 cells are an imprecise model for mast cell mediator
RT   release.";
RL   Inflamm. Res. 58:611-618(2009).
//
RX   PubMed=19669619; DOI=10.1007/s00011-009-0074-y;
RA   Passante E., Frankish N.;
RT   "The RBL-2H3 cell line: its provenance and suitability as a model for
RT   the mast cell.";
RL   Inflamm. Res. 58:737-745(2009).
//
RX   PubMed=25149487; DOI=10.1007/978-1-4939-1173-8_8;
RA   Passante E.;
RT   "Mast cell and basophil cell lines: a compendium.";
RL   Methods Mol. Biol. 1192:101-113(2014).
//
RX   PubMed=36125550; DOI=10.1007/s00441-022-03687-w;
RA   Rujitharanawong C., Yoodee S., Sueksakit K., Peerapen P., Tuchinda P.,
RA   Kulthanan K., Thongboonkerd V.;
RT   "Systematic comparisons of various markers for mast cell activation in
RT   RBL-2H3 cells.";
RL   Cell Tissue Res. 390:413-428(2022).
//