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Cellosaurus PA-1 (CVCL_0479)

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Cell line name PA-1
Synonyms PA1; PA I; PAI
Accession CVCL_0479
Resource Identification Initiative To cite this cell line use: PA-1 (RRID:CVCL_0479)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: KuDOS 95 cell line panel.
Doubling time: 36 hours (PubMed=2653399); ~24 hours (Note=Lot 01212002) (JCRB=JCRB9061); 22.77 hours (JWGray panel).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Caution: TP53 mutation indicated to be at p.Asn239Asp (c.715A>G) in DOI=10.11418/jtca1981.16.3_173 and PubMed=9359923.
Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous; Note=Not found in early passages (PubMed=6740333).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Pro316Pro (c.948C>T); ClinVar=VCV000750727; Zygosity=Heterozygous (Cosmic-CLP=909255).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*68:02,68:02
HLA-BB*37:04,44:03
HLA-CC*07:01,07:01
Class II
HLA-DQDQB1*04:01,04:01
HLA-DRDRB1*03:02,03:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African21.08
Native American17.69
East Asian, North1.54
East Asian, South0.7
South Asian0.4
European, North26.01
European, South32.58
Disease Ovarian mixed germ cell tumor (NCIt: C8114)
Mixed germ cell tumor (ORDO: Orphanet_180234)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_1T15 (222)CVCL_4992 (CH1 [Human ovarian carcinoma])CVCL_3121 (PA-1/6TG-r)
CVCL_E6ZI (PA1 fat-1)
Sex of cell Female
Age at sampling 12Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-1572; CCRID=1101HUM-PUMC000021; CLS=300402; COG; Cosmic-CLP=909255; DepMap=ACH-001374; ECACC=90013101; JCRB=JCRB9061; PubMed=25877200

Markers:
AmelogeninX
CSF1PO9,12
D2S133824
D3S135815
D5S81811
D7S8209
D8S117914,15
D13S3179,10 (ATCC=CRL-1572; CCRID=1101HUM-PUMC000021; CLS=300402; COG; Cosmic-CLP=909255; DepMap=ACH-001374; ECACC=90013101; PubMed=25877200)
9,10,13 (JCRB=JCRB9061)
D16S5399 (CLS=300402)
9,12 (ATCC=CRL-1572; CCRID=1101HUM-PUMC000021; COG; Cosmic-CLP=909255; DepMap=ACH-001374; ECACC=90013101; JCRB=JCRB9061; PubMed=25877200)
D18S5115,18
D19S43313
D21S1129,31.2
FGA24
Penta D9,12
Penta E14,20
TH017,9
TPOX11
vWA15,17

Run an STR similarity search on this cell line
Publications

PubMed=6931103; DOI=10.1002/ijc.2910250104
Jesper Zeuthen, Jan O. Rytter Norgaard, Philip Avner, Marc Fellous, Jorma Wartiovaara, Antti Vaheri, Anders Rosen, Beppino Carlo Giovanella;
Characterization of a human ovarian teratocarcinoma-derived cell line.
Int. J. Cancer 25:19-32(1980)

PubMed=6740333; DOI=10.1126/science.6740333
Michael A. Tainsky, Colin S. Cooper, Beppino Carlo Giovanella, George F. Vande Woude Jr.;
An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells.
Science 225:643-645(1984)

PubMed=2653399; DOI=10.1038/bjc.1989.108; PMCID=PMC2247140
Celia A. Hills, Lloyd R. Kelland, George Abel, Jan Siracky, Anne P. Wilson, Kenneth Reginald Harrap;
Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes.
Br. J. Cancer 59:527-534(1989)

DOI=10.11418/jtca1981.16.3_173
Koichiro Mihara, Masahiro Miyazaki, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Ryuichiro Ohashi, Masayoshi Namba;
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9359923; DOI=10.18926/AMO/30789
Koichiro Mihara, Masahiro Miyazaki, Tadashi Kondo, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Chikashi Ishioka, Masayoshi Namba;
Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.
Acta Med. Okayama 51:261-265(1997)

PubMed=9698466; DOI=10.1006/gyno.1998.5039
George Larry Maxwell, John I. Risinger, Betty Tong, Heather Shaw, J. Carl Barrett, Andrew Berchuck, P. Andrew Futreal;
Mutation of the PTEN tumor suppressor gene is not a feature of ovarian cancers.
Gynecol. Oncol. 70:13-16(1998)

PubMed=10567668; DOI=10.3892/ijmm.4.6.597
Chong Gao, Masahiro Miyazaki, Jun-Wu Li, Toshiya Tsuji, Yusuke Inoue, Masayoshi Namba;
Cytogenetic characteristics and p53 gene status of human teratocarcinoma PA-1 cells in 407-445 passages.
Int. J. Mol. Med. 4:597-600(1999)

PubMed=11314036; DOI=10.1038/sj.onc.1204211
Eva Forgacs, Jonathan D. Wren, Craig Kamibayashi, Masashi Kondo, Xie L. Xu, Sanford David Markowitz, Gail E. Tomlinson, Carolyn Y. Muller, Adi F. Gazdar, Harold Ray Garner, John D. Minna;
Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.
Oncogene 20:1005-1009(2001)

PubMed=11330945; DOI=10.1006/gyno.2001.6132
Takafumi Watanabe, Issei Imoto, Yoshinori Kosugi, Isamu Ishiwata, Satoshi Inoue, Masaomi Takayama, Akira Sato, Johji Inazawa;
A novel amplification at 17q21-23 in ovarian cancer cell lines detected by comparative genomic hybridization.
Gynecol. Oncol. 81:172-177(2001)

PubMed=15677628; DOI=10.1093/carcin/bgi032
Kylie Louise Gorringe, Suet-Feung Chin, Paul D.P. Pharoah, Joanne M. Staines, Carla Oliveira, Paul A.W. Edwards, Carlos Caldas;
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)

PubMed=16080959; DOI=10.1016/j.cancergencyto.2005.01.003
Shireen A. Sarraf, Raphael Tejada, Massih Abawi, Michael Oberst, Tom Dennis, Kelly Claire Simon, Jan K. Blancato;
The human ovarian teratocarcinoma cell line PA-1 demonstrates a single translocation: analysis with fluorescence in situ hybridization, spectral karyotyping, and bacterial artificial chromosome microarray.
Cancer Genet. Cytogenet. 161:63-69(2005)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017; PMCID=PMC3432677
Christopher T. Korch, Monique A. Spillman, Twila A. Jackson, Britta M. Jacobsen, Susan K. Murphy, Bruce Arthur Lessey, Virgil Craig Jordan, Andrew Peter Bradford;
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=23415752; DOI=10.1016/j.molonc.2012.12.007; PMCID=PMC4106023
Britta Stordal, Kirsten Timms, Angela Farrelly, Danielle Gallagher, Steven Busschots, Mickael Renaud, Julien Thery, Deborah Williams, Jennifer Potter, Thanh Tran, Greg Korpanty ...Show all 19 authors... , Mattia Cremona, Mark S. Carey, Jie Li, Yang Li, Ozlem Aslan, John J. O'Leary, Gordon B. Mills, Bryan T. Hennessy; Show fewer authors
BRCA1/2 mutation analysis in 41 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation.
Mol. Oncol. 7:567-579(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-1572
BCRC; 60063
CLS; 300402
ECACC; 90013101
ICLC; HTL97002
IZSLER; BS TCL 179
JCRB; JCRB9061
RCB; RCB1946 - Discontinued
TKG; TKG 0573 - Discontinued
Cell line databases/resources CLO; CLO_0008350
MCCL; MCC:0000377
CLDB; cl3856
CLDB; cl3857
CLDB; cl3858
CLDB; cl3859
cancercelllines; CVCL_0479
CCRID; 1101HUM-PUMC000021
Cell_Model_Passport; SIDM01140
CGH-DB; 9358-4
Cosmic-CLP; 909255
DepMap; ACH-001374
LINCS_LDP; LCL-1416
Anatomy/cell type resources BTO; BTO_0001591
Biological sample resources BioSample; SAMN01821586
BioSample; SAMN03472415
BioSample; SAMN03473209
Chemistry resources ChEMBL-Cells; CHEMBL3308812
ChEMBL-Targets; CHEMBL614949
GDSC; 909255
PharmacoDB; PA1_1235_2019
PubChem_Cell_line; CVCL_0479
Encyclopedic resources Wikidata; Q54937331
Experimental variables resources EFO; EFO_0006465
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-3610
FANTOM5_SSTAR; 10807-111A6
GEO; GSM186444
GEO; GSM186445
GEO; GSM784566
GEO; GSM1374804
GEO; GSM1670330
Polymorphism and mutation databases Cosmic; 688112
Cosmic; 801353
Cosmic; 809111
Cosmic; 844343
Cosmic; 909255
Cosmic; 931368
Cosmic; 1093560
Cosmic; 1305331
Cosmic; 1995610
IARC_TP53; 1418
IARC_TP53; 8338
Progenetix; CVCL_0479
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number47