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Cellosaurus MEG-01 (CVCL_0425)

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Cell line name MEG-01
Synonyms Meg-01; MEG01; Meg01
Accession CVCL_0425
Resource Identification Initiative To cite this cell line use: MEG-01 (RRID:CVCL_0425)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Characteristics: Can be induced to differentiate into megakaryocytes after application of 12-O-tetradecanoyl-13-phorbol acetate (TPA) or valproic acid (VPA) (PubMed=1742484; PubMed=30332548).
Doubling time: 36-48 hours (PubMed=2998511); ~35 hours (DSMZ=ACC-364).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Misspelling: Mego-1; Note=Occasionally.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 13 fused to ABL1 exon 2 (b2a2 transcript) (PubMed=10071072; PubMed=10576511; PubMed=15843827; PubMed=20809971; DepMap=ACH-000072).
  • Mutation; HGNC; HGNC:18318; ASXL1; Simple; p.Gly646Trpfs*12 (c.1934dupG); ClinVar=VCV000426927; Zygosity=Heterozygous (PubMed=26700326; DepMap=ACH-000072).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.His233del (c.697_699delCAC); Zygosity=Heterozygous (Cosmic-CLP=908126; DepMap=ACH-000072).
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.14
Native American0
East Asian, North79.13
East Asian, South17.4
South Asian0
European, North0
European, South2.33
Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_E2Q6 (HyCyte MEG-01 hJAK2_p.V617F_c.1849G>T)CVCL_3022 (MEG-01s)CVCL_VG38 (SPYMEG)
Sex of cell Male
Age at sampling 55Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2021; Cosmic-CLP=908126; DSMZ=ACC-364; ECACC=94012401; JCRB=IFO50151

Markers:
AmelogeninX,Y
CSF1PO10
D2S133819
D3S135815
D5S81813
D7S82011
D8S117914,15
D13S3178
D16S5399
D18S5118,22
D19S43314,16
D21S1129
FGA26
Penta D11,13
Penta E15
TH017
TPOX8,11
vWA16

Run an STR similarity search on this cell line
Web pages Info; KAKEN; 60480281; https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480281/
Publications

PubMed=2998511; DOI=10.1182/blood.V66.6.1384.1384
Michinori Ogura, Yasuo Morishima, Ryuzo Ohno, Yukio Kato, Norio Hirabayashi, Hiroshi Nagura, Hidehiko Saito;
Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome.
Blood 66:1384-1392(1985)

PubMed=3332852; DOI=10.1016/S0950-3536(87)80037-9
Armand Keating;
Ph positive CML cell lines.
Baillieres Clin. Haematol. 1:1021-1029(1987)

PubMed=1742484; DOI=10.1182/blood.V78.12.3168.3168
Takashi Murate, Tomomitsu Hotta, Keitaro Tsushita, Motoshi Suzuki, Tomoaki Yoshida, Shinsuke Saga, Hidehiko Saito, Shonen Yoshida;
Aphidicolin, an inhibitor of DNA replication, blocks the TPA-induced differentiation of a human megakaryoblastic cell line, MEG-O1.
Blood 78:3168-3177(1991)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886
Jun Takizawa, Ritsuro Suzuki, Hiroyuki Kuroda, Atae Utsunomiya, Yoshitoyo Kagami, Tatsuroh Joh, Yoshifusa Aizawa, Ryuzo Ueda, Masao Seto;
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10071072; DOI=10.1016/S0145-2126(98)00171-4
Hans Gunther Drexler, Roderick A.F. MacLeod, Cord C. Uphoff;
Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.
Leuk. Res. 23:207-215(1999)

PubMed=10576511; DOI=10.1016/S0145-2126(99)00131-9
Cord C. Uphoff, Silke Habig, Severine Fombonne, Yoshinobu Matsuo, Hans Gunther Drexler;
ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.
Leuk. Res. 23:1055-1060(1999)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Anna K. Hagstrom-Andersson, Patrik Eden, David Lindgren, Jens Nilsson, Carin Lassen, Jesper Heldrup, Magnus Fontes, Ake Borg, Felix Mitelman, Bertil Johansson, Mattias Hoglund, Thoas Fioretos;
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20809971; DOI=10.1186/1755-8166-3-15; PMCID=PMC2944125
Anna Virgili, Elisabeth Nacheva;
Genomic amplification of BCR/ABL1 and a region downstream of ABL1 in chronic myeloid leukaemia: a FISH mapping study of CML patients and cell lines.
Mol. Cytogenet. 3:15.1-15.12(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=26700326; DOI=10.1016/j.exphem.2015.11.011
Daichi Inoue, Masaki Matsumoto, Reina Nagase, Makoto Saika, Takeshi Fujino, Keiichi I. Nakayama, Toshio Kitamura;
Truncation mutants of ASXL1 observed in myeloid malignancies are expressed at detectable protein levels.
Exp. Hematol. 44:172-176.e1(2016)

PubMed=27277069; DOI=10.1160/TH15-11-0891; PMCID=PMC5080539
Joy R. Wright, Stefan Amisten, Alison H. Goodall, Martyn P. Mahaut-Smith;
Transcriptomic analysis of the ion channelome of human platelets and megakaryocytic cell lines.
Thromb. Haemost. 116:272-284(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786
Kar-Tong Tan, Ling-Wen Ding, Qiao-Yang Sun, Zhen-Tang Lao, Wenwen Chien, Xi Ren, Jin-Fen Xiao, Xin-Yi Loh, Liang Xu, Michael Lill, Anand Mayakonda ...Show all 14 authors... , De-Chen Lin, Henry He Yang, Harold Phillip Koeffler; Show fewer authors
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30332548; DOI=10.1080/09537104.2018.1528344
Ankita Dhenge, Rutuja Kuhikar, Vaijayanti Kale, Lalita Shrikant Limaye;
Regulation of differentiation of MEG01 to megakaryocytes and platelet-like particles by valproic acid through Notch3 mediated actin polymerization.
Platelets 30:780-795(2019)

PubMed=30629668; DOI=10.1371/journal.pone.0210404; PMCID=PMC6328144
Cord C. Uphoff, Claudia Pommerenke, Sabine A. Denkmann, Hans Gunther Drexler;
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:e0210404.1-e0210404.22(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=32180119; DOI=10.1007/s12185-020-02853-6
Hiroshi Saito, Morisada Hayakawa, Nobuhiko Kamoshita, Atsushi Yasumoto, Katsue Suzuki-Inoue, Yutaka Yatomi, Tsukasa Ohmori;
Establishment of a megakaryoblastic cell line for conventional assessment of platelet calcium signaling.
Int. J. Hematol. 111:786-794(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=36982453; DOI=10.3390/ijms24065377; PMCID=PMC10049680
Rafal Skopek, Malgorzata Palusinska, Katarzyna Kaczor-Keller, Rafal Pingwara, Anna Papierniak-Wygladala, Tino Schenk, Slawomir Lewicki, Artur Zelent, Lukasz Szymanski;
Choosing the right cell line for acute myeloid leukemia (AML) research.
Int. J. Mol. Sci. 24:5377.1-5377.34(2023)

Cross-references
Cell line collections (Providers) ATCC; CRL-2021
BCRC; 60238
BCRJ; 0262
CLS; 300482
DSMZ; ACC-364
ECACC; 94012401
ICLC; HTL96017
JCRB; IFO50151
Cell line databases/resources CLO; CLO_0007668
MCCL; MCC:0000322
CLDB; cl3432
CLDB; cl3433
cancercelllines; CVCL_0425
CCRID; 1101HUM-PUMC000521
CCRID; 3101HUMTCHu197
Cell_Model_Passport; SIDM00526
Cosmic-CLP; 908126
DepMap; ACH-000072
DSMZCellDive; ACC-364
IGRhCellID; MEG01
LINCS_LDP; LCL-1110
Lonza; 959
Anatomy/cell type resources BTO; BTO_0002581
Biological sample resources BioSample; SAMN01821577
BioSample; SAMN01821648
BioSample; SAMN01821734
BioSample; SAMN03471278
BioSample; SAMN10988184
Chemistry resources ChEMBL-Cells; CHEMBL3308539
ChEMBL-Targets; CHEMBL2366084
GDSC; 908126
PharmacoDB; MEG01_913_2019
PubChem_Cell_line; CVCL_0425
Encyclopedic resources Wikidata; Q54905035
Experimental variables resources EFO; EFO_0002232
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
FANTOM5_SSTAR; 10752-110D5
GEO; GSM482498
GEO; GSM887307
GEO; GSM888383
GEO; GSM1374669
GEO; GSM1670093
Polymorphism and mutation databases Cosmic; 787503
Cosmic; 908126
Cosmic; 924042
Cosmic; 1026578
Cosmic; 1070702
Cosmic; 1078723
Cosmic; 1516634
Cosmic; 1523830
IARC_TP53; 21491
LiGeA; CCLE_381
Progenetix; CVCL_0425
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number46