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Cellosaurus MC3T3-E1 (CVCL_0409)

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Cell line name MC3T3-E1
Synonyms Mc3T3-E1; MC3T3E1; MC-3T3-E1; MC 3T3-E1
Accession CVCL_0409
Resource Identification Initiative To cite this cell line use: MC3T3-E1 (RRID:CVCL_0409)
Comments Group: Space-flown cell line (cellonaut).
Doubling time: ~1-2 days (DSMZ=ACC-210).
Omics: Variations; SNP array analysis.
Anecdotal: Have been flown in space on shuttle flights STS-56, STS-76, STS-81 and STS-84 to study growth and differentiation in microgravity (PubMed=8612673; PubMed=12638602).
Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.
Cell type: Osteoblast; CL=CL_0000062.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0D74 (MC3T3)
Children:
CVCL_VT93 (MC3T3-E1 Subclone 11)CVCL_5437 (MC3T3-E1 Subclone 14)CVCL_VT94 (MC3T3-E1 Subclone 17)
CVCL_VT95 (MC3T3-E1 Subclone 20)CVCL_5438 (MC3T3-E1 Subclone 24)CVCL_VT96 (MC3T3-E1 Subclone 26)
CVCL_5439 (MC3T3-E1 Subclone 30)CVCL_VT97 (MC3T3-E1 Subclone 35)CVCL_5440 (MC3T3-E1 Subclone 4)
CVCL_VT98 (MC3T3-E1 Subclone 8)CVCL_B6H0 (MC3T3-NM4)
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
STR profile Source(s): PubMed=37712227

Markers:
Mouse STR 4-220.3
Mouse STR 5-517
Mouse STR 6-717
Mouse STR 12-117
Mouse STR 15-322.3
Mouse STR 18-315
Mouse STR X-128

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-393.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/MC3T3
Publications

DOI=10.2330/joralbiosci1965.23.899
Hiro-aki Kodama, Yuji Amagai, Hiroko Sudo, Shiro Kasai, Shigehisa Yamamoto;
Establishment of a clonal osteogenic cell line from newborn mouse calvaria.
Jpn. J. Oral Biol. 23:899-901(1981)

PubMed=6826647; DOI=10.1083/jcb.96.1.191; PMCID=PMC2112252
Hiroko Sudo, Hiro-aki Kodama, Yuji Amagai, Shigehisa Yamamoto, Shiro Kasai;
In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.
J. Cell Biol. 96:191-198(1983)

PubMed=8612673; DOI=10.1006/excr.1996.0116
Millie Elizabeth Hughes-Fulford, Marian L. Lewis;
Effects of microgravity on osteoblast growth activation.
Exp. Cell Res. 224:103-109(1996)

PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893
Dian Wang, Kurt Christensen, Kanwal Chawla, Guo-Zhi Xiao, Paul H. Krebsbach, Renny Theodore Franceschi;
Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential.
J. Bone Miner. Res. 14:893-903(1999)

PubMed=11136541; DOI=10.1007/s002230001157
Totomi T. Baba;
Restoration of mineral depositions by dexamethasone in the matrix of nonmineralizing osteoblastic cells subcloned from MC3T3-E1 cells.
Calcif. Tissue Int. 67:416-421(2000)

PubMed=12638602
Millie Elizabeth Hughes-Fulford;
Changes in gene expression and signal transduction in microgravity.
J. Gravit. Physiol. 8:P1-P4(2001)

DOI=10.4051/ibc.2010.2.4.0014
Kaori Yoshino, Kaoru Saijo, Chikako Noro, Yukio Nakamura;
Development of a simple method to determine the mouse strain from which cultured cell lines originated.
Interdisciplinary Bio Cent. 2:14.1-14.9(2010)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
John Paul Didion, Ryan J. Buus, Zohreh Naghashfar, David W. Threadgill, Herbert Carpenter Morse 3rd, Fernando Pardo-Manuel de Villena;
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=37712227; DOI=10.1242/dmm.050150; PMCID=PMC10618599
Yi-Hsien Chen, Jon P. Connelly, Colin P. Florian, Xiao-Xia Cui, Shondra M. Pruett-Miller;
Short tandem repeat profiling via next-generation sequencing for cell line authentication.
Dis. Model. Mech. 16:dmm050150.1-dmm050150.10(2023)

Cross-references
Cell line collections (Providers) ATCC; CRL-2953 - Discontinued
CCTCC; GDC0188
DSMZ; ACC-210
ECACC; 99072810
RCB; RCB1126
Cell line databases/resources CLO; CLO_0007582
CLO; CLO_0050208
MCCL; MCC:0000303
CLDB; cl40
CCRID; 1101MOU-PUMC000012
DSMZCellDive; ACC-210
FCS-free; 185-5-353-1-3-3
Lonza; 521
Anatomy/cell type resources BTO; BTO_0001957
Chemistry resources ChEMBL-Cells; CHEMBL4295391
ChEMBL-Targets; CHEMBL4296463
PubChem_Cell_line; CVCL_0409
Encyclopedic resources Wikidata; Q54904201
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number30