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Cellosaurus MC3T3-E1 (CVCL_0409)

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Cell line name MC3T3-E1
Synonyms Mc3T3-E1; MC3T3E1; MC-3T3-E1; MC 3T3-E1
Accession CVCL_0409
Resource Identification Initiative To cite this cell line use: MC3T3-E1 (RRID:CVCL_0409)
Comments Group: Space-flown cell line (cellonaut).
Doubling time: ~1-2 days (DSMZ=ACC-210).
Omics: SNP array analysis.
Anecdotal: Have been flown in space on shuttle flights STS-56, STS-76, STS-81 and STS-84 to study growth and differentiation in microgravity (PubMed=8612673; PubMed=12638602).
Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.
Cell type: Osteoblast; CL=CL_0000062.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0D74 (MC3T3)
CVCL_VT93 (MC3T3-E1 Subclone 11)CVCL_5437 (MC3T3-E1 Subclone 14)CVCL_VT94 (MC3T3-E1 Subclone 17)
CVCL_VT95 (MC3T3-E1 Subclone 20)CVCL_5438 (MC3T3-E1 Subclone 24)CVCL_VT96 (MC3T3-E1 Subclone 26)
CVCL_5439 (MC3T3-E1 Subclone 30)CVCL_VT97 (MC3T3-E1 Subclone 35)CVCL_5440 (MC3T3-E1 Subclone 4)
CVCL_VT98 (MC3T3-E1 Subclone 8)CVCL_B6H0 (MC3T3-NM4)
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/MC3T3

Kodama H.-A., Amagai Y., Sudo H., Kasai S., Yamamoto S.
Establishment of a clonal osteogenic cell line from newborn mouse calvaria.
Jpn. J. Oral Biol. 23:899-901(1981)

PubMed=6826647; DOI=10.1083/jcb.96.1.191
Sudo H., Kodama H.-A., Amagai Y., Yamamoto S., Kasai S.
In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.
J. Cell Biol. 96:191-198(1983)

PubMed=8612673; DOI=10.1006/excr.1996.0116
Hughes-Fulford M., Lewis M.L.
Effects of microgravity on osteoblast growth activation.
Exp. Cell Res. 224:103-109(1996)

PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893
Wang D., Christensen K., Chawla K., Xiao G.-Z., Krebsbach P.H., Franceschi R.T.
Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential.
J. Bone Miner. Res. 14:893-903(1999)

PubMed=11136541; DOI=10.1007/s002230001157
Baba T.T.
Restoration of mineral depositions by dexamethasone in the matrix of nonmineralizing osteoblastic cells subcloned from MC3T3-E1 cells.
Calcif. Tissue Int. 67:416-421(2000)

Hughes-Fulford M.
Changes in gene expression and signal transduction in microgravity.
J. Gravit. Physiol. 8:P1-P4(2001)

Yoshino K., Saijo K., Noro C., Nakamura Y.
Development of a simple method to determine the mouse strain from which cultured cell lines originated.
Interdisciplinary Bio Central 2:1-9(2010)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

Cell line collections (Providers) ATCC; CRL-2953 - Discontinued
ECACC; 99072810
RCB; RCB1126
Cell line databases/resources CLO; CLO_0007582
CLO; CLO_0050208
MCCL; MCC:0000303
CLDB; cl40
CCRID; 1101MOU-PUMC000012
DSMZCellDive; ACC-210
FCS-free; 185-5-353-1-3-3
Lonza; 521
Anatomy/cell type resources BTO; BTO:0001957
Chemistry resources ChEMBL-Cells; CHEMBL4295391
ChEMBL-Targets; CHEMBL4296463
PubChem_Cell_line; CVCL_0409
Encyclopedic resources Wikidata; Q54904201
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number27