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Cellosaurus MC3T3-E1 Subclone 14 (CVCL_5437)

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Cell line name MC3T3-E1 Subclone 14
Synonyms MC3T3-E1 SUBCLONE 14
Accession CVCL_5437
Resource Identification Initiative To cite this cell line use: MC3T3-E1 Subclone 14 (RRID:CVCL_5437)
Comments Characteristics: Exhibits high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. Forms a well mineralized extracellular matrix (ECM) after 10 days (ATCC=CRL-2594).
Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.
Cell type: Osteoblast; CL=CL_0000062.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0409 (MC3T3-E1)
Children:
CVCL_C8WY (MC3T3-E1 Subclone 14-Luc)CVCL_E0Y9 (Ubigene MC3T3-E1 Subclone 14 Clk4 KO)CVCL_E0YA (Ubigene MC3T3-E1 Subclone 14 Egfr KO)
CVCL_E0YB (Ubigene MC3T3-E1 Subclone 14 Notum KO)CVCL_E0YC (Ubigene MC3T3-E1 Subclone 14 Npnt KO)CVCL_E0YD (Ubigene MC3T3-E1 Subclone 14 Tsc1 KO)
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
STR profile Source(s): Ubigene=YC-C026

Markers:
Mouse STR 4-220.3
Mouse STR 5-517
Mouse STR 6-418
Mouse STR 6-717
Mouse STR 12-117
Mouse STR 15-322.3
Mouse STR 18-315
Mouse STR X-128

Run an STR similarity search on this cell line
Publications

PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893
Dian Wang, Kurt Christensen, Kanwal Chawla, Guo-Zhi Xiao, Paul H. Krebsbach, Renny Theodore Franceschi;
Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential.
J. Bone Miner. Res. 14:893-903(1999)

Cross-references
Cell line collections (Providers) ATCC; CRL-2594
BCRJ; 0285
CLS; 305185
KCB; KCB 200786YJ
Ubigene; YC-C026
Cell line databases/resources CLO; CLO_0007583
CCRID; 3101MOUGNM15
Encyclopedic resources Wikidata; Q54904202
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number17