ID   MC3T3-E1
AC   CVCL_0409
SY   Mc3T3-E1; MC3T3E1; MC-3T3-E1; MC 3T3-E1
DR   BTO; BTO_0001957
DR   CLO; CLO_0007582
DR   CLO; CLO_0050208
DR   MCCL; MCC:0000303
DR   CLDB; cl40
DR   ATCC; CRL-2953
DR   CCRID; 1101MOU-PUMC000012
DR   CCTCC; GDC0188
DR   ChEMBL-Cells; CHEMBL4295391
DR   ChEMBL-Targets; CHEMBL4296463
DR   DSMZ; ACC-210
DR   DSMZCellDive; ACC-210
DR   ECACC; 99072810
DR   FCS-free; 185-5-353-1-3-3
DR   Lonza; 521
DR   PubChem_Cell_line; CVCL_0409
DR   RCB; RCB1126
DR   Wikidata; Q54904201
RX   DOI=10.2330/joralbiosci1965.23.899;
RX   DOI=10.4051/ibc.2010.2.4.0014;
RX   PubMed=6826647;
RX   PubMed=8612673;
RX   PubMed=10352097;
RX   PubMed=11136541;
RX   PubMed=12638602;
RX   PubMed=25277546;
RX   PubMed=37712227;
WW   Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-393.html
WW   Info; Wikipedia; -; https://en.wikipedia.org/wiki/MC3T3
CC   Group: Space-flown cell line (cellonaut).
CC   Doubling time: ~1-2 days (DSMZ=ACC-210).
CC   Omics: Variations; SNP array analysis.
CC   Anecdotal: Have been flown in space on shuttle flights STS-56, STS-76, STS-81 and STS-84 to study growth and differentiation in microgravity (PubMed=8612673; PubMed=12638602).
CC   Discontinued: ATCC; CRL-2953; true.
CC   Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.
CC   Cell type: Osteoblast; CL=CL_0000062.
CC   Breed/subspecies: C57BL/6.
ST   Source(s): PubMed=37712227
ST   Mouse STR 4-2: 20.3
ST   Mouse STR 5-5: 17
ST   Mouse STR 6-7: 17
ST   Mouse STR 12-1: 17
ST   Mouse STR 15-3: 22.3
ST   Mouse STR 18-3: 15
ST   Mouse STR X-1: 28
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0D74 ! MC3T3
SX   Sex unspecified
AG   <1D
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 30
//
RX   DOI=10.2330/joralbiosci1965.23.899;
RA   Kodama H.-a., Amagai Y., Sudo H., Kasai S., Yamamoto S.;
RT   "Establishment of a clonal osteogenic cell line from newborn mouse
RT   calvaria.";
RL   Jpn. J. Oral Biol. 23:899-901(1981).
//
RX   DOI=10.4051/ibc.2010.2.4.0014;
RA   Yoshino K., Saijo K., Noro C., Nakamura Y.;
RT   "Development of a simple method to determine the mouse strain from which
RT   cultured cell lines originated.";
RL   Interdisciplinary Bio Central 2:1-9(2010).
//
RX   PubMed=6826647; DOI=10.1083/jcb.96.1.191; PMCID=PMC2112252;
RA   Sudo H., Kodama H.-a., Amagai Y., Yamamoto S., Kasai S.;
RT   "In vitro differentiation and calcification in a new clonal osteogenic
RT   cell line derived from newborn mouse calvaria.";
RL   J. Cell Biol. 96:191-198(1983).
//
RX   PubMed=8612673; DOI=10.1006/excr.1996.0116;
RA   Hughes-Fulford M.E., Lewis M.L.;
RT   "Effects of microgravity on osteoblast growth activation.";
RL   Exp. Cell Res. 224:103-109(1996).
//
RX   PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893;
RA   Wang D., Christensen K., Chawla K., Xiao G.-Z., Krebsbach P.H.,
RA   Franceschi R.T.;
RT   "Isolation and characterization of MC3T3-E1 preosteoblast subclones
RT   with distinct in vitro and in vivo differentiation/mineralization
RT   potential.";
RL   J. Bone Miner. Res. 14:893-903(1999).
//
RX   PubMed=11136541; DOI=10.1007/s002230001157;
RA   Baba T.T.;
RT   "Restoration of mineral depositions by dexamethasone in the matrix of
RT   nonmineralizing osteoblastic cells subcloned from MC3T3-E1 cells.";
RL   Calcif. Tissue Int. 67:416-421(2000).
//
RX   PubMed=12638602;
RA   Hughes-Fulford M.E.;
RT   "Changes in gene expression and signal transduction in microgravity.";
RL   J. Gravit. Physiol. 8:P1-P4(2001).
//
RX   PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738;
RA   Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W.,
RA   Morse H.C. 3rd, Pardo-Manuel de Villena F.;
RT   "SNP array profiling of mouse cell lines identifies their strains of
RT   origin and reveals cross-contamination and widespread aneuploidy.";
RL   BMC Genomics 15:847.1-847.11(2014).
//
RX   PubMed=37712227; DOI=10.1242/dmm.050150; PMCID=PMC10618599;
RA   Chen Y.-H., Connelly J.P., Florian C.P., Cui X.-X.,
RA   Pruett-Miller S.M.;
RT   "Short tandem repeat profiling via next-generation sequencing for cell
RT   line authentication.";
RL   Dis. Model. Mech. 16:dmm050150.1-dmm050150.10(2023).
//