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Cellosaurus JEG-3 (CVCL_0363)

[Text version]

Cell line name JEG-3
Synonyms Jeg-3; JEG3; Jeg3; jeg3
Accession CVCL_0363
Resource Identification Initiative To cite this cell line use: JEG-3 (RRID:CVCL_0363)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Characteristics: Established by serial cloning into hamster cheek pouches.
Doubling time: ~24 hours (DSMZ); ~36 hours (CLS).
Karyotypic information: Near tetraploid karyotype (PubMed=33096371).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Caution: A TP53 mutation is indicated as being at p.Gln167His (c.501G>T) in PubMed=7819056 but has not been confirmed by CCLE and Cosmic-CLP.
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0.09
East Asian, North0.97
East Asian, South0
South Asian0.9
European, North71.88
European, South26.16
Disease Gestational choriocarcinoma (NCIt: C4646)
Gestational choriocarcinoma (ORDO: Orphanet_99926)
Derived from metastatic site: Fetal brain.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_9559 (AC1 [Human gestational choriocarcinoma] )CVCL_IV65 (JEG-3/A3-1A7 )CVCL_6922 (JEG-3/VP16 )
CVCL_7167 (TEC61 )
Originate from same individual CVCL_0044 ! BeWo
Sex of cell Male
Age at sampling Fetus
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CCRID; CLS; Cosmic-CLP; DepMap; DSMZ; KCLB; PubMed=33810901

D3S135815 (CLS)
15,16 (CCRID; DepMap; DSMZ; KCLB; PubMed=33810901)
Penta D9,12
Penta E8,12

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/j/cell-lines-detail-251.html

PubMed=5103722; DOI=10.1210/jcem-32-5-683
Kohler P.O., Bridson W.E.
Isolation of hormone-producing clonal lines of human choriocarcinoma.
J. Clin. Endocrinol. Metab. 32:683-687(1971)

PubMed=62839; DOI=10.1093/jnci/56.5.911
Lieblich J.M., Weintraub B.D., Krauth G.H., Kohler P.O., Rabson A.S., Rosen S.W.
Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro: comparison of clonal strains from carcinomas of lung and placenta.
J. Natl. Cancer Inst. 56:911-917(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=2878840; DOI=10.1016/0020-7292(86)90088-3
Yamashita K., Nakamura T., Shimizu T., Katagiri M., Ikeda H.
Expression of HLA class I and class II antigens in human choriocarcinoma cell lines.
Int. J. Gynecol. Obstet. 24:301-307(1986)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=7937597; DOI=10.1016/0143-4004(94)90008-6
Grummer R., Hohn H.-P., Mareel M.M., Denker H.-W.
Adhesion and invasion of three human choriocarcinoma cell lines into human endometrium in a three-dimensional organ culture system.
Placenta 15:411-429(1994)

Brown A.D., Lopez-Terrada D., Denny C., Lee K.A.W.
Promoters containing ATF-binding sites are de-regulated in cells that express the EWS/ATF1 oncogene.
Oncogene 10:1749-1756(1995)

PubMed=7819056; DOI=10.1038/bjc.1995.3
Yaginuma Y., Yamashita T., Takuma N., Katayama H., Ishikawa M.
Analysis of the p53 gene in human choriocarcinoma cell lines.
Br. J. Cancer 71:9-12(1995)

Ganapathy V., Prasad P.D., Leibach F.H.
(In) Human cell culture. Vol. 2. Cancer Cell Lines part 2; Masters J.R.W., Palsson B.O. (eds.); pp.141-147; Kluwer Academic Publishers; New York (1999)

PubMed=11994546; DOI=10.1093/molehr/8.5.485
Blanchon L., Sauvant P., Bavik C., Gallot D., Charbonne F., Alexandre-Gouabau M.-C., Lemery D., Jacquetin B., Dastugue B., Ward S., Sapin V.
Human choriocarcinoma cell line JEG-3 produces and secretes active retinoids from retinol.
Mol. Hum. Reprod. 8:485-493(2002)

PubMed=12966428; DOI=10.1038/sj.bjc.6601212
Li K.K.C., Goodall J., Goding C.R., Liao S.-K., Wang C.-H., Lin Y.-C., Hiraga H., Nojima T., Nagashima K., Schaefer K.-L., Lee K.A.W.
The melanocyte inducing factor MITF is stably expressed in cell lines from human clear cell sarcoma.
Br. J. Cancer 89:1072-1078(2003)

PubMed=16797695; DOI=10.1016/j.placenta.2006.05.001
Burleigh D.W., Kendziorski C.M., Choi Y.J., Grindle K.M., Grendell R.L., Magness R.R., Golos T.G.
Microarray analysis of BeWo and JEG3 trophoblast cell lines: identification of differentially expressed transcripts.
Placenta 28:383-389(2007)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28161053; DOI=10.1016/j.placenta.2016.12.007
Abou-Kheir W., Barrak J., Hadadeh O., Daoud G.
HTR-8/SVneo cell line contains a mixed population of cells.
Placenta 50:1-7(2017)

PubMed=28724925; DOI=10.1038/s41598-017-06364-z
Rothbauer M., Patel N., Gondola H., Siwetz M., Huppertz B., Ertl P.
A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines.
Sci. Rep. 7:5892-5892(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=33096371; DOI=10.1016/j.placenta.2020.10.011
Weber M., Weise A., Vasheghani F., Gohner C., Fitzgerald J.S., Liehr T., Markert U.R.
Cytogenomics of six human trophoblastic cell lines.
Placenta 103:72-75(2021)

PubMed=33810901; DOI=10.1016/j.placenta.2021.02.022
Pastuschek J., Nonn O., Gutierrez-Samudio R.N., Murrieta-Coxca J.M., Muller J., Sanft J., Huppertz B., Markert U.R., Groten T., Morales-Prieto D.M.
Molecular characteristics of established trophoblast-derived cell lines.
Placenta 108:122-133(2021)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cell line collections AddexBio; C0030003/4940
BCRC; 60428
BCRJ; 0123
CLS; 300222
ECACC; 92120308
KCLB; 30036
NCBI_Iran; C544
Cell line databases/resources CLO; CLO_0007017
MCCL; MCC:0000257
CLDB; cl2935
CLDB; cl2936
CCRID; 1101HUM-PUMC000071
Cell_Model_Passport; SIDM01218
Cosmic-CLP; 907176
DepMap; ACH-001530
DSMZCellDive; ACC-463
TOKU-E; 2070
Anatomy/cell type resources BTO; BTO:0002269
Biological sample resources BioSample; SAMN01821538
BioSample; SAMN01821638
Chemistry resources ChEMBL-Cells; CHEMBL3308202
ChEMBL-Targets; CHEMBL2366348
GDSC; 907176
PharmacoDB; JEG3_677_2019
Encyclopedic resources Wikidata; Q54898563
Experimental variables resources EFO; EFO_0002066
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM48266
GEO; GSM48267
GEO; GSM48268
GEO; GSM1669948
Polymorphism and mutation databases Cosmic; 683654
Cosmic; 907176
Cosmic; 1312288
Cosmic; 2077192
IARC_TP53; 4344
IARC_TP53; 21418
Progenetix; CVCL_0363
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update22-Sep-2022
Version number39