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Cellosaurus BeWo (CVCL_0044)

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Cell line name BeWo
Synonyms BEWO; Be Wo; Be-Wo
Accession CVCL_0044
Resource Identification Initiative To cite this cell line use: BeWo (RRID:CVCL_0044)
Comments Part of: Human Protein Atlas, Cell Atlas panel.
Population: Caucasian.
Characteristics: Cells were initially transplanted to the cheek pouch of a hamster and maintained by serial passage for 8 years prior to the establishment of the cell line.
Doubling time: ~30 hours (DSMZ=ACC-458).
Karyotypic information: Near triploid karyotype (PubMed=33096371).
Omics: Proteomics; Expression; Large antibody panel staining analysis.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Fetal brain; UBERON=UBERON_0006238.
HLA typing Source: IPD-IMGT/HLA=10312
Class I
HLA-CC*04:01:01:01,07:01
Disease Gestational choriocarcinoma (NCIt: C4646)
Gestational choriocarcinoma (ORDO: Orphanet_99926)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_LB82 (BeWo b24)CVCL_LB83 (BeWo b30)CVCL_XF88 (Bewo31)
CVCL_R702 (Hs 630.T)CVCL_E1JD (HyCyte Bewo KO-hABCC1)CVCL_E1JE (HyCyte Bewo KO-hABCG2)
CVCL_D8ES (Ubigene BeWo ERVFRD-1 KO)CVCL_D8ET (Ubigene BeWo TRPV6 KO)
Originate from same individual CVCL_0363 ! JEG-3
Sex of cell Male
Age at sampling Fetus
Category Cancer cell line
STR profile Source(s): AddexBio=C0030001/4905; ATCC=CCL-98; CCRID; CLS=300123; DSMZ=ACC-458; ECACC=86082803; IZSLER=BS TCL 3; KCLB=10098; PubMed=33810901; RCB=RCB1644; TKG=TKG 0567

Markers:
AmelogeninX,Y
CSF1PO11,12
D1S165614,16
D2S44114
D2S133821,24
D3S135815
D5S81810,11
D7S82010,12
D8S117912
D10S124813,15
D12S39117,24
D13S3179,11
D16S53913,14
D18S5114,16
D19S43313,15
D21S1130
D22S104515
FGA22,23 (KCLB=10098)
22,23,24 (ATCC=CCL-98; CCRID; CLS=300123; DSMZ=ACC-458; PubMed=33810901)
Penta D9,12
Penta E8,12
SE3315,25.2
TH019,9.3
TPOX8
vWA16

Run an STR similarity search on this cell line
Web pages Info; -; -; https://web.archive.org/web/20141030051820/http://www2.kumc.edu/soalab/LabLinks/recipes/bewo.htm
Info; HPA; -; https://www.proteinatlas.org/learn/cellines
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/b/cell-lines-detail-163.html
Publications

PubMed=14207855; DOI=10.1093/jnci/33.3.441
Clyde J. Dawe, William G. Banfield 2nd, Willie DeWard Morgan, Marilyn Shaw Slatick, Helen Ollendorf-Curth;
Growth in continuous culture, and in hamsters, of cells from a neoplasm associated with acanthosis nigricans.
J. Natl. Cancer Inst. 33:441-456(1964)

PubMed=4299001
Roland Anthony Pattillo, George Otto Gey;
The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.
Cancer Res. 28:1231-1236(1968)

PubMed=5753554; DOI=10.1126/science.159.3822.1467
Roland Anthony Pattillo, George Otto Gey, Eleanor Mary Delfs, Richard F. Mattingly;
Human hormone production in vitro.
Science 159:1467-1469(1968)

PubMed=6988327; DOI=10.1007/BF02831503
Stephen James O'Brien, John E. Shannon, Mitchell H. Gail;
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=6174649
Souei Sekiya, Makoto Kawata, Noriyuki Inaba, Hiroyoshi Takamizawa;
Studies on marker substances in cell lines derived from various human gynecologic tumors.
Nihon Sanka Fujinka Gakkai Zasshi 34:37-42(1982)

PubMed=7153601
Shigeru Sasaki, Paul K. Katayama, Mark Roesler, Roland Anthony Pattillo, Richard F. Mattingly, Kimiyasu Ohkawa;
Cytogenetic analysis of choriocarcinoma cell lines.
Nihon Sanka Fujinka Gakkai Zasshi 34:2253-2256(1982)

PubMed=6682628; DOI=10.1016/0002-9378(83)90927-4
Souei Sekiya, Teruyo Kaiho, Syoichi Shirotake, Hiroshi Iwasawa, Bin Takeda, Hiroyoshi Takamizawa;
Effect of methotrexate on the growth and human chorionic gonadotropin secretion of human choriocarcinoma cell lines in vitro.
Am. J. Obstet. Gynecol. 146:57-64(1983)

PubMed=4038916; DOI=10.1016/0165-4608(85)90052-4
David M. Sheppard, Rosemary A. Fisher, Sylvia Dorothy Lawler;
Karyotypic analysis and chromosome polymorphisms in four choriocarcinoma cell lines.
Cancer Genet. Cytogenet. 16:251-258(1985)

PubMed=2878840; DOI=10.1016/0020-7292(86)90088-3
Kohki Yamashita, Takafumi Nakamura, Tetsuya Shimizu, Makoto Katagiri, Hisami Ikeda;
Expression of HLA class I and class II antigens in human choriocarcinoma cell lines.
Int. J. Gynaecol. Obstet. 24:301-307(1986)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=2720636; DOI=10.1016/0165-4608(89)90664-X
Urvashi Surti, Rezvan Habibian;
Chromosomal rearrangement in choriocarcinoma cell lines.
Cancer Genet. Cytogenet. 38:229-240(1989)

PubMed=7937597; DOI=10.1016/0143-4004(94)90008-6
Ruth Grummer, Hans-Peter Hohn, Marc M. Mareel, Hans-Werner Denker;
Adhesion and invasion of three human choriocarcinoma cell lines into human endometrium in a three-dimensional organ culture system.
Placenta 15:411-429(1994)

PubMed=7819056; DOI=10.1038/bjc.1995.3; PMCID=PMC2033447
Yuji Yaginuma, Tsuyoshi Yamashita, Naoyuki Takuma, Hideto Katayama, Mutsuo Ishikawa;
Analysis of the p53 gene in human choriocarcinoma cell lines.
Br. J. Cancer 71:9-12(1995)

DOI=10.1007/0-306-46861-1_8
Vadivel Ganapathy, Puttur D. Prasad, Frederick H. Leibach;
Choriocarcinoma.
(In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.141-147; Kluwer Academic Publishers; New York; USA (1999)

PubMed=11897413; DOI=10.1016/S0378-5173(01)00929-2
Chadarat Ampasavate, Gurudatt A. Chandorkar, David G. Vander Velde, John F. Stobaugh, Kenneth L. Audus;
Transport and metabolism of opioid peptides across BeWo cells, an in vitro model of the placental barrier.
Int. J. Pharm. 233:85-98(2002)

PubMed=16957166; DOI=10.1369/jhc.6A7001.2006; PMCID=PMC3958123
Ann-Catrin Andersson, Sara Stromberg, Helena Backvall, Caroline Kampf, Mathias Uhlen, Kenneth Wester, Fredrik Ponten;
Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics.
J. Histochem. Cytochem. 54:1413-1423(2006)

PubMed=16797695; DOI=10.1016/j.placenta.2006.05.001
David W. Burleigh, Christina Marie Kendziorski, Yun Jung Choi, Kreg M. Grindle, Richard L. Grendell, Ronald R. Magness, Thaddeus G. Golos;
Microarray analysis of BeWo and JEG3 trophoblast cell lines: identification of differentially expressed transcripts.
Placenta 28:383-389(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera, Rui Wang, Angela Tam ...Show all 22 authors... , Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=28161053; DOI=10.1016/j.placenta.2016.12.007
Wassim Abou-Kheir, Joanna Barrak, Ola Hadadeh, Georges Daoud;
HTR-8/SVneo cell line contains a mixed population of cells.
Placenta 50:1-7(2017)

PubMed=32178868; DOI=10.1016/j.bbrc.2020.03.031
Zhuo-Si Li, Osamu Kurosawa, Hiroo Iwata;
A comparative study of key physiological stem cell parameters between three human trophoblast cell lines.
Biochem. Biophys. Res. Commun. 525:1038-1045(2020)

PubMed=33096371; DOI=10.1016/j.placenta.2020.10.011
Maja Weber, Anja Weise, Faezeh Vasheghani, Claudia Gohner, Justine Sayuri Fitzgerald, Thomas Liehr, Udo R. Markert;
Cytogenomics of six human trophoblastic cell lines.
Placenta 103:72-75(2021)

PubMed=33810901; DOI=10.1016/j.placenta.2021.02.022
Jana Pastuschek, Olivia Nonn, Ruby N. Gutierrez-Samudio, Jose Martin Murrieta-Coxca, Jasmin Muller, Juliane Sanft, Berthold Huppertz, Udo R. Markert, Tanja Groten, Diana M. Morales-Prieto;
Molecular characteristics of established trophoblast-derived cell lines.
Placenta 108:122-133(2021)

Cross-references
Cell line collections (Providers) AddexBio; C0030001/4905
ATCC; CCL-98
BCRC; 60073
BCRJ; 0048
CCTCC; GDC0072
CLS; 300123
DSMZ; ACC-458
ECACC; 86082803
IZSLER; BS TCL 3
JCRB; JCRB9111
KCLB; 10098
RCB; RCB1644
RCB; RCB3678
TKG; TKG 0567
Ubigene; YC-D018
Cell line databases/resources CLO; CLO_0001934
CLO; CLO_0001935
CLO; CLO_0050014
CLDB; cl415
CLDB; cl416
cancercelllines; CVCL_0044
CCRID; 1101HUM-PUMC000156
CCRID; 4201HUM-CCTCC00072
Cell_Model_Passport; SIDM00692
DSMZCellDive; ACC-458
IGRhCellID; BeWo
IPD-IMGT/HLA; 10312
LINCS_LDP; LCL-1293
Lonza; 1449
TOKU-E; 661
Anatomy/cell type resources BTO; BTO_0000798
Biological sample resources BioSample; SAMN01821537
BioSample; SAMN01821617
BioSample; SAMN03471293
Chemistry resources ChEMBL-Cells; CHEMBL3308359
ChEMBL-Targets; CHEMBL614525
PubChem_Cell_line; CVCL_0044
Encyclopedic resources Wikidata; Q54796111
Experimental variables resources EFO; EFO_0002050
Gene expression databases ArrayExpress; E-MTAB-38
FANTOM5_SSTAR; 10423-106C9
GEO; GSM48269
GEO; GSM48270
GEO; GSM48271
GEO; GSM48272
GEO; GSM48273
GEO; GSM7701452
GEO; GSM7701456
Polymorphism and mutation databases IARC_TP53; 21200
Progenetix; CVCL_0044
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number45