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Cellosaurus Hs 294T (CVCL_0331)

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Cell line name Hs 294T
Synonyms HS-294T; Hs-294T; HS-294-T; HS-294; Hs294T; HS294T; Hs294; HS294
Accession CVCL_0331
Resource Identification Initiative To cite this cell line use: Hs 294T (RRID:CVCL_0331)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Misspelling: HTB-140; Note=Based on the ATCC catalog number.
Misspelling: HTB140; Note=Based on the ATCC catalog number.
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000029.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (PubMed=15467732; DepMap=ACH-000014).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,25:01
HLA-BB*07:02,08:01
HLA-CC*07:02,07:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.39
East Asian, North0
East Asian, South0
South Asian0.34
European, North72.19
European, South27.07
Disease Melanoma (NCIt: C3224)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_1057 (A101D)CVCL_E3AI (Hs294T R)
Sex of cell Male
Age at sampling 56Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-140; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11
D2S133817,24
D3S135816,17
D5S81812
D7S82010
D8S117913,14 (ATCC=HTB-140)
14 (PubMed=25877200)
D13S31711,12
D16S53911,12
D18S5113,17
D19S43314,14.2
D21S1130
FGA23,24
Penta D13
Penta E7,10
TH018,9.3
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Publications

PubMed=265522; DOI=10.1073/pnas.74.2.565; PMCID=PMC392331
Robert Neal Fabricant, Joseph E. De Larco, George J. Todaro;
Nerve growth factor receptors on human melanoma cells in culture.
Proc. Natl. Acad. Sci. U.S.A. 74:565-569(1977)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Jorgen Fogh, Jens M. Fogh, Thomas Orfeo;
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=375235; DOI=10.1073/pnas.76.3.1288; PMCID=PMC383236
Stephen A. Sherwin, Ann H. Sliski, George J. Todaro;
Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces.
Proc. Natl. Acad. Sci. U.S.A. 76:1288-1292(1979)

PubMed=478563; DOI=10.1007/BF02616140
Abla A. Creasey, Helene S. Smith, Adeline Johnson Hackett, Kimie Fukuyama, William L. Epstein, Stewart Harvey Madin;
Biological properties of human melanoma cells in culture.
In Vitro 15:342-350(1979)

PubMed=6220172
Nicholas C. Dracopoli, Jorgen Fogh;
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3335022
Michael C. Alley, Dominic A. Scudiero, Anne Monks, Miriam L. Hursey, Maciej J. Czerwinski, Donald L. Fine, Betty Jane Abbott, Joseph G. Mayo, Robert H. Shoemaker, Michael R. Boyd;
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)

PubMed=8755562; DOI=10.1073/pnas.93.15.7832; PMCID=PMC38834
Da-Cai Liu, Eric Pearlman, Eugenia Diaconu, Kun Guo, Hiroshi Mori, Tariq Haqqi, Sanford David Markowitz, James K.V. Willson, Man-Sun Sy;
Expression of hyaluronidase by tumor cells induces angiogenesis in vivo.
Proc. Natl. Acad. Sci. U.S.A. 93:7832-7837(1996)

PubMed=11414198; DOI=10.1007/s004320000207
Harald Lahm, Sabine Andre, Andreas Hoeflich, Jurgen R. Fischer, Bernard Sordat, Herbert Kaltner, Eckhard Wolf, Hans-Joachim Gabius;
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=15467732; DOI=10.1038/sj.onc.1208152
Hideaki Tanami, Issei Imoto, Akira Hirasawa, Yasuhiro Yuki, Itaru Sonoda, Jun Inoue, Kohichiroh Yasui, Akiko Misawa-Furihata, Yutaka Kawakami, Johji Inazawa;
Involvement of overexpressed wild-type BRAF in the growth of malignant melanoma cell lines.
Oncogene 23:8796-8804(2004)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=39175042; DOI=10.1186/s12964-024-01788-3; PMCID=PMC11342534
Magdalena Kot, Aleksandra Simiczyjew, Justyna Wadzynska, Marcin Zietek, Rafa Adam Matkowski, Dorota Nowak;
Characterization of two melanoma cell lines resistant to BRAF/MEK inhibitors (vemurafenib and cobimetinib).
Cell Commun. Signal. 22:410.1-410.21(2024)

Cross-references
Cell line collections (Providers) ATCC; HTB-140
Cell line databases/resources CLO; CLO_0003917
MCCL; MCC:0000227
cancercelllines; CVCL_0331
Cell_Model_Passport; SIDM00800
CGH-DB; 9319-4
DepMap; ACH-000014
Anatomy/cell type resources BTO; BTO_0003467
Biological sample resources BioSample; SAMN03471454
BioSample; SAMN10988350
CRISP screens repositories BioGRID_ORCS_Cell_line; 178
Chemistry resources ChEMBL-Cells; CHEMBL4523539
ChEMBL-Targets; CHEMBL4523570
PharmacoDB; Hs294T_573_2019
PubChem_Cell_line; CVCL_0331
Encyclopedic resources Wikidata; Q54895578
Experimental variables resources EFO; EFO_0006585
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
GEO; GSE52246
GEO; GSM206511
GEO; GSM274685
GEO; GSM887096
GEO; GSM888167
Polymorphism and mutation databases Cosmic; 905232
Cosmic; 933000
IARC_TP53; 30047
LiGeA; CCLE_572
Progenetix; CVCL_0331
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number46