ID   Hs 294T
AC   CVCL_0331
SY   HS-294T; Hs-294T; HS-294-T; HS-294; Hs294T; HS294T; Hs294; HS294
DR   BTO; BTO_0003467
DR   CLO; CLO_0003917
DR   EFO; EFO_0006585
DR   MCCL; MCC:0000227
DR   ArrayExpress; E-MTAB-2706
DR   ArrayExpress; E-MTAB-2770
DR   ATCC; HTB-140
DR   BioGRID_ORCS_Cell_line; 178
DR   BioSample; SAMN03471454
DR   BioSample; SAMN10988350
DR   cancercelllines; CVCL_0331
DR   Cell_Model_Passport; SIDM00800
DR   CGH-DB; 9319-4
DR   ChEMBL-Cells; CHEMBL4523539
DR   ChEMBL-Targets; CHEMBL4523570
DR   Cosmic; 905232
DR   Cosmic; 933000
DR   DepMap; ACH-000014
DR   EGA; EGAS00001000610
DR   GEO; GSE52246
DR   GEO; GSM206511
DR   GEO; GSM274685
DR   GEO; GSM887096
DR   GEO; GSM888167
DR   IARC_TP53; 30047
DR   LiGeA; CCLE_572
DR   PharmacoDB; Hs294T_573_2019
DR   Progenetix; CVCL_0331
DR   PubChem_Cell_line; CVCL_0331
DR   Wikidata; Q54895578
RX   PubMed=265522;
RX   PubMed=327080;
RX   PubMed=375235;
RX   PubMed=478563;
RX   PubMed=3335022;
RX   PubMed=3518877;
RX   PubMed=6220172;
RX   PubMed=8755562;
RX   PubMed=11414198;
RX   PubMed=15467732;
RX   PubMed=22460905;
RX   PubMed=25485619;
RX   PubMed=25877200;
RX   PubMed=26589293;
RX   PubMed=30894373;
RX   PubMed=31068700;
RX   PubMed=31978347;
RX   PubMed=39175042;
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Population: Caucasian.
CC   HLA typing: A*01:01,25:01; B*07:02,08:01; C*07:02,07:02 (PubMed=26589293).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (PubMed=15467732; DepMap=ACH-000014).
CC   Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
CC   Omics: Genomics; Whole exome sequencing.
CC   Omics: Proteomics; Quantitative.
CC   Omics: Transcriptomics; Microarray.
CC   Omics: Transcriptomics; RNAseq.
CC   Omics: Variations; SNP array analysis.
CC   Genome ancestry: African=0%; Native American=0.39%; East Asian, North=0%; East Asian, South=0%; South Asian=0.34%; European, North=72.19%; European, South=27.07% (PubMed=30894373).
CC   Misspelling: HTB-140; Note=Based on the ATCC catalog number.
CC   Misspelling: HTB140; Note=Based on the ATCC catalog number.
CC   Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000029.
ST   Source(s): ATCC=HTB-140; PubMed=25877200
ST   Amelogenin: X
ST   CSF1PO: 11
ST   D13S317: 11,12
ST   D16S539: 11,12
ST   D18S51: 13,17
ST   D19S433: 14,14.2
ST   D21S11: 30
ST   D2S1338: 17,24
ST   D3S1358: 16,17
ST   D5S818: 12
ST   D7S820: 10
ST   D8S1179: 13,14 (ATCC=HTB-140)
ST   D8S1179: 14 (PubMed=25877200)
ST   FGA: 23,24
ST   Penta D: 13
ST   Penta E: 7,10
ST   TH01: 8,9.3
ST   TPOX: 8,11
ST   vWA: 16,18
DI   NCIt; C3224; Melanoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   56Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 46
CH   CVCL_1057 ! A101D
CH   CVCL_E3AI ! Hs294T R
//
RX   PubMed=6220172;
RA   Dracopoli, Nicholas C.
RA   Fogh, Jorgen
RT   "Polymorphic enzyme analysis of cultured human tumor cell lines.";
RL   J. Natl. Cancer Inst. 70:469-476(1983).
//
RX   PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027;
RA   Barretina, Jordi Ginesta
RA   Caponigro, Giordano
RA   Stransky, Nicolas
RA   Venkatesan, Kavitha
RA   Margolin, Adam A.
RA   Kim, Sungjoon
RA   Wilson, Christopher J.
RA   Lehar, Joseph
RA   Kryukov, Gregory V.
RA   Sonkin, Dmitriy
RA   Reddy, Anupama
RA   Liu, Manway
RA   Murray, Lauren
RA   Berger, Michael F.
RA   Monahan, John E.
RA   Morais, Paula
RA   Meltzer, Jodi
RA   Korejwa, Adam
RA   Jane-Valbuena, Judit
RA   Mapa, Felipa A.
RA   Thibault, Joseph
RA   Bric-Furlong, Eva
RA   Raman, Pichai
RA   Shipway, Aaron
RA   Engels, Ingo H.
RA   Cheng, Jill
RA   Yu, Guo-Ying K.
RA   Yu, Jian-Jun
RA   Aspesi, Peter Jr.
RA   de Silva, Melanie
RA   Jagtap, Kalpana
RA   Jones, Michael D.
RA   Wang, Li
RA   Hatton, Charles
RA   Palescandolo, Emanuele
RA   Gupta, Supriya
RA   Mahan, Scott
RA   Sougnez, Carrie
RA   Onofrio, Robert C.
RA   Liefeld, Ted
RA   MacConaill, Laura Eleanor
RA   Winckler, Wendy
RA   Reich, Michael
RA   Li, Nan-Xin
RA   Mesirov, Jill P.
RA   Gabriel, Stacey B.
RA   Getz, Gad
RA   Ardlie, Kristin
RA   Chan, Vivien
RA   Myer, Vic E.
RA   Weber, Barbara L.
RA   Porter, Jeff
RA   Warmuth, Markus
RA   Finan, Peter
RA   Harris, Jennifer L.
RA   Meyerson, Matthew Langer
RA   Golub, Todd Robert
RA   Morrissey, Michael P.
RA   Sellers, William Raj
RA   Schlegel, Robert
RA   Garraway, Levi Alexander
RT   "The Cancer Cell Line Encyclopedia enables predictive modelling of
RT   anticancer drug sensitivity.";
RL   Nature 483:603-607(2012).
//
RX   PubMed=3518877; DOI=10.3109/07357908609038260;
RA   Fogh, Jorgen
RT   "Human tumor lines for cancer research.";
RL   Cancer Invest. 4:157-184(1986).
//
RX   PubMed=39175042; DOI=10.1186/s12964-024-01788-3; PMCID=PMC11342534;
RA   Kot, Magdalena
RA   Simiczyjew, Aleksandra
RA   Wadzynska, Justyna
RA   Zietek, Marcin
RA   Matkowski, Rafa Adam
RA   Nowak, Dorota
RT   "Characterization of two melanoma cell lines resistant to BRAF/MEK
RT   inhibitors (vemurafenib and cobimetinib).";
RL   Cell Commun. Signal. 22:410.1-410.21(2024).
//
RX   PubMed=375235; DOI=10.1073/pnas.76.3.1288; PMCID=PMC383236;
RA   Sherwin, Stephen A.
RA   Sliski, Ann H.
RA   Todaro, George J.
RT   "Human melanoma cells have both nerve growth factor and nerve growth
RT   factor-specific receptors on their cell surfaces.";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:1288-1292(1979).
//
RX   PubMed=327080; DOI=10.1093/jnci/59.1.221;
RA   Fogh, Jorgen
RA   Fogh, Jens M.
RA   Orfeo, Thomas
RT   "One hundred and twenty-seven cultured human tumor cell lines
RT   producing tumors in nude mice.";
RL   J. Natl. Cancer Inst. 59:221-226(1977).
//
RX   PubMed=25877200; DOI=10.1038/nature14397;
RA   Yu, Ma-Mie
RA   Selvaraj, Suresh K.
RA   Liang-Chu, May M.Y.
RA   Aghajani, Sahar
RA   Busse, Matthew
RA   Yuan, Jean
RA   Lee, Genee
RA   Peale, Franklin V.
RA   Klijn, Christiaan
RA   Bourgon, Richard
RA   Kaminker, Joshua S.
RA   Neve, Richard M.
RT   "A resource for cell line authentication, annotation and quality
RT   control.";
RL   Nature 520:307-311(2015).
//
RX   PubMed=25485619; DOI=10.1038/nbt.3080;
RA   Klijn, Christiaan
RA   Durinck, Steffen
RA   Stawiski, Eric W.
RA   Haverty, Peter M.
RA   Jiang, Zhao-Shi
RA   Liu, Han-Bin
RA   Degenhardt, Jeremiah
RA   Mayba, Oleg
RA   Gnad, Florian
RA   Liu, Jin-Feng
RA   Pau, Gregoire
RA   Reeder, Jens
RA   Cao, Yi
RA   Mukhyala, Kiran
RA   Selvaraj, Suresh K.
RA   Yu, Ma-Mie
RA   Zynda, Gregory J.
RA   Brauer, Matthew J.
RA   Wu, Thomas D.
RA   Gentleman, Robert Clifford
RA   Manning, Gerard
RA   Yauch, Robert L.
RA   Bourgon, Richard
RA   Stokoe, David
RA   Modrusan, Zora
RA   Neve, Richard M.
RA   de Sauvage, Frederic J.
RA   Settleman, Jeffrey
RA   Seshagiri, Somasekar
RA   Zhang, Ze-Min
RT   "A comprehensive transcriptional portrait of human cancer cell
RT   lines.";
RL   Nat. Biotechnol. 33:306-312(2015).
//
RX   PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675;
RA   Dutil, Julie
RA   Chen, Zhi-Hua
RA   Monteiro, Alvaro N.A.
RA   Teer, Jamie K.
RA   Eschrich, Steven A.
RT   "An interactive resource to probe genetic diversity and estimated
RT   ancestry in cancer cell lines.";
RL   Cancer Res. 79:1263-1273(2019).
//
RX   PubMed=478563; DOI=10.1007/BF02616140;
RA   Creasey, Abla A.
RA   Smith, Helene S.
RA   Hackett, Adeline Johnson
RA   Fukuyama, Kimie
RA   Epstein, William L.
RA   Madin, Stewart Harvey
RT   "Biological properties of human melanoma cells in culture.";
RL   In Vitro 15:342-350(1979).
//
RX   PubMed=11414198; DOI=10.1007/s004320000207;
RA   Lahm, Harald
RA   Andre, Sabine
RA   Hoeflich, Andreas
RA   Fischer, Jurgen R.
RA   Sordat, Bernard
RA   Kaltner, Herbert
RA   Wolf, Eckhard
RA   Gabius, Hans-Joachim
RT   "Comprehensive galectin fingerprinting in a panel of 61 human tumor
RT   cell lines by RT-PCR and its implications for diagnostic and
RT   therapeutic procedures.";
RL   J. Cancer Res. Clin. Oncol. 127:375-386(2001).
//
RX   PubMed=265522; DOI=10.1073/pnas.74.2.565; PMCID=PMC392331;
RA   Fabricant, Robert Neal
RA   De Larco, Joseph E.
RA   Todaro, George J.
RT   "Nerve growth factor receptors on human melanoma cells in culture.";
RL   Proc. Natl. Acad. Sci. U.S.A. 74:565-569(1977).
//
RX   PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254;
RA   Nusinow, David Paul
RA   Szpyt, John
RA   Ghandi, Mahmoud
RA   Rose, Christopher M.
RA   McDonald, E. Robert 3rd
RA   Kalocsay, Marian
RA   Jane-Valbuena, Judit
RA   Gelfand, Ellen T.
RA   Schweppe, Devin Karl
RA   Jedrychowski, Mark P.
RA   Golji, Javad
RA   Porter, Dale A.
RA   Rejtar, Tomas
RA   Wang, Y. Karen
RA   Kryukov, Gregory V.
RA   Stegmeier, Frank
RA   Erickson, Brian K.
RA   Garraway, Levi Alexander
RA   Sellers, William Raj
RA   Gygi, Steven P.
RT   "Quantitative proteomics of the Cancer Cell Line Encyclopedia.";
RL   Cell 180:387-402.e16(2020).
//
RX   PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103;
RA   Ghandi, Mahmoud
RA   Huang, Franklin W.
RA   Jane-Valbuena, Judit
RA   Kryukov, Gregory V.
RA   Lo, Christopher C.
RA   McDonald, E. Robert 3rd
RA   Barretina, Jordi Ginesta
RA   Gelfand, Ellen T.
RA   Bielski, Craig M.
RA   Li, Hao-Xin
RA   Hu, Kevin
RA   Andreev-Drakhlin, Alexander Y.
RA   Kim, Jaegil
RA   Hess, Julian M.
RA   Haas, Brian J.
RA   Aguet, Francois
RA   Weir, Barbara A.
RA   Rothberg, Michael V.
RA   Paolella, Brenton R.
RA   Lawrence, Michael Scott
RA   Akbani, Rehan
RA   Lu, Yi-Ling
RA   Tiv, Hong L.
RA   Gokhale, Prafulla C.
RA   de Weck, Antoine
RA   Mansour, Ali Amin
RA   Oh, Coyin
RA   Shih, Juliann
RA   Hadi, Kevin
RA   Rosen, Yanay
RA   Bistline, Jonathan
RA   Venkatesan, Kavitha
RA   Reddy, Anupama
RA   Sonkin, Dmitriy
RA   Liu, Manway
RA   Lehar, Joseph
RA   Korn, Joshua M.
RA   Porter, Dale A.
RA   Jones, Michael D.
RA   Golji, Javad
RA   Caponigro, Giordano
RA   Taylor, Jordan E.
RA   Dunning, Caitlin M.
RA   Creech, Amanda L.
RA   Warren, Allison C.
RA   McFarland, James M.
RA   Zamanighomi, Mahdi
RA   Kauffmann, Audrey
RA   Stransky, Nicolas
RA   Imielinski, Marcin
RA   Maruvka, Yosef E.
RA   Cherniack, Andrew D.
RA   Tsherniak, Aviad
RA   Vazquez, Francisca
RA   Jaffe, Jacob D.
RA   Lane, Andrew Alan
RA   Weinstock, David M.
RA   Johannessen, Cory M.
RA   Morrissey, Michael P.
RA   Stegmeier, Frank
RA   Schlegel, Robert
RA   Hahn, William Chun
RA   Getz, Gad
RA   Mills, Gordon B.
RA   Boehm, Jesse S.
RA   Golub, Todd Robert
RA   Garraway, Levi Alexander
RA   Sellers, William Raj
RT   "Next-generation characterization of the Cancer Cell Line
RT   Encyclopedia.";
RL   Nature 569:503-508(2019).
//
RX   PubMed=3335022;
RA   Alley, Michael Curtis
RA   Scudiero, Dominic A.
RA   Monks, Anne
RA   Hursey, Miriam L.
RA   Czerwinski, Maciej J.
RA   Fine, Donald L.
RA   Abbott, Betty Jane
RA   Mayo, Joseph G.
RA   Shoemaker, Robert H.
RA   Boyd, Michael R.
RT   "Feasibility of drug screening with panels of human tumor cell lines
RT   using a microculture tetrazolium assay.";
RL   Cancer Res. 48:589-601(1988).
//
RX   PubMed=15467732; DOI=10.1038/sj.onc.1208152;
RA   Tanami, Hideaki
RA   Imoto, Issei
RA   Hirasawa, Akira
RA   Yuki, Yasuhiro
RA   Sonoda, Itaru
RA   Inoue, Jun
RA   Yasui, Kohichiroh
RA   Misawa-Furihata, Akiko
RA   Kawakami, Yutaka
RA   Inazawa, Johji
RT   "Involvement of overexpressed wild-type BRAF in the growth of
RT   malignant melanoma cell lines.";
RL   Oncogene 23:8796-8804(2004).
//
RX   PubMed=8755562; DOI=10.1073/pnas.93.15.7832; PMCID=PMC38834;
RA   Liu, Da-Cai
RA   Pearlman, Eric
RA   Diaconu, Eugenia
RA   Guo, Kun
RA   Mori, Hiroshi
RA   Haqqi, Tariq
RA   Markowitz, Sanford David
RA   Willson, James K.V.
RA   Sy, Man-Sun
RT   "Expression of hyaluronidase by tumor cells induces angiogenesis in
RT   vivo.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:7832-7837(1996).
//
RX   PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878;
RA   Scholtalbers, Jelle
RA   Boegel, Sebastian
RA   Bukur, Thomas
RA   Byl, Marius
RA   Goerges, Sebastian
RA   Sorn, Patrick
RA   Loewer, Martin
RA   Sahin, Ugur
RA   Castle, John C.
RT   "TCLP: an online cancer cell line catalogue integrating HLA type,
RT   predicted neo-epitopes, virus and gene expression.";
RL   Genome Med. 7:118.1-118.7(2015).
//