Cellosaurus logo
expasy logo

Cellosaurus HuCC-T1 (CVCL_0324)

[Text version]
Cell line name HuCC-T1
Synonyms HuCCT-1; HUCCT-1; HUCC-T1; HUCCT1; HuCCT1
Accession CVCL_0324
Resource Identification Initiative To cite this cell line use: HuCC-T1 (RRID:CVCL_0324)
Comments Part of: Biliary tract cancer cell line atlas.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: TCGA-110-CL cell line panel.
Population: Japanese.
Doubling time: ~55 hours (Note=At 10th passage in RPMI + 10% FBS), ~74 hours (Note=At 25th passage in RPMI + 0.2% LA) (PubMed=2544546).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; HLA class I peptidome.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (Cosmic-CLP=907069; DepMap=ACH-000976).
  • Mutation; HGNC; HGNC:7329; MSH6; Simple; p.Lys1358fs*2 (c.4071_4072insGATT); Zygosity=Heterozygous (Cosmic-CLP=907069; DepMap=ACH-000976).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Homozygous (Cosmic-CLP=907069; DepMap=ACH-000976).
Genome ancestry Source: PubMed=30894373

Origin% genome
African7.42
Native American0
East Asian, North82
East Asian, South0
South Asian0
European, North0
European, South10.57
Disease Intrahepatic cholangiocarcinoma (NCIt: C35417)
Cholangiocarcinoma (ORDO: Orphanet_70567)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_QZ44 (HuCC-T1-G10)CVCL_QZ45 (HuCC-T1-G100)
Sex of cell Male
Age at sampling 56Y
Category Cancer cell line
STR profile Source(s): CLS=300469; Cosmic-CLP=907069; JCRB=JCRB0425; PubMed=25877200; RCB=RCB1960; TKG=TKG 0389

Markers:
AmelogeninX,Y
CSF1PO11,12
D2S133817,18
D3S135815
D5S81812,13
D6S104313
D7S82010,11
D8S117910
D12S39118,20
D13S31711,13
D16S5399,11,12 (PubMed=25877200)
11,12 (CLS=300469; Cosmic-CLP=907069; JCRB=JCRB0425; RCB=RCB1960; TKG=TKG 0389)
D18S5113
D19S43313
D21S1131
FGA20,23
Penta D10
Penta E15,18
TH017,10
TPOX8
vWA18

Run an STR similarity search on this cell line
Publications

PubMed=2544546; DOI=10.1007/BF02623562
Miki Miyagiwa, Takafumi Ichida, Takayoshi Tokiwa, Jiro Sato, Hiroshi Sasaki;
A new human cholangiocellular carcinoma cell line (HuCC-T1) producing carbohydrate antigen 19/9 in serum-free medium.
In Vitro Cell. Dev. Biol. 25:503-510(1989)

DOI=10.11418/jtca1981.16.3_173
Koichiro Mihara, Masahiro Miyazaki, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Ryuichiro Ohashi, Masayoshi Namba;
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21451941; DOI=10.1007/s00534-011-0376-7
Jun Sato, Takashi Kimura, Takuro Saito, Takayuki Anazawa, Akira Kenjo, Yoshihiro Sato, Takao Tsuchiya, Mitsukazu Gotoh;
Gene expression analysis for predicting gemcitabine resistance in human cholangiocarcinoma.
J. Hepatobiliary Pancreat. Sci. 18:700-711(2011)

PubMed=21687941; DOI=10.3892/ijo.2011.1087
Michihiro Kamigaki, Tamito Sasaki, Masahiro Serikawa, Motoki Inoue, Kenso Kobayashi, Hiroshi Itsuki, Tomoyuki Minami, Masanobu Yukutake, Akihito Okazaki, Takashi Ishigaki, Yasutaka Ishii ...Show all 13 authors... , Keiichi Kosaka, Kazuaki Chayama; Show fewer authors
Statins induce apoptosis and inhibit proliferation in cholangiocarcinoma cells.
Int. J. Oncol. 39:561-568(2011)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

CLPUB00275
Sebastian Zach, Emrullah Birgin, Felix Ruckert;
Primary cholangiocellular carcinoma cell lines.
J. Stem Cell Res. Transplant. 2:1013-1013(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26956050; DOI=10.18632/oncotarget.7914; PMCID=PMC4991440
Xi-Wei Ding, Roongruedee Chaiteerakij, Catherine D. Moser, Hassan M. Shaleh, Jeffrey Boakye, Gang Chen, Albert Ndzengue, Ying Li, Yan-Ling Zhou, Sheng-Bing Huang, Frank A. Sinicrope ...Show all 15 authors... , Xiao-Ping Zou, Melanie Byrne Thomas, Charles D. Smith, Lewis Rowland Roberts; Show fewer authors
Antitumor effect of the novel sphingosine kinase 2 inhibitor ABC294640 is enhanced by inhibition of autophagy and by sorafenib in human cholangiocarcinoma cells.
Oncotarget 7:20080-20092(2016)

PubMed=27231123; DOI=10.1158/2159-8290.CD-15-1442; PMCID=PMC5458737
Supriya Kumar Saha, John D. Gordan, Benjamin P. Kleinstiver, Phuong Vu, Mortada S. Najem, Jia-Chi Yeo, Lei Shi, Yasutaka Kato, Rebecca S. Levin, James T. Webber, Leah J. Damon ...Show all 28 authors... , Regina K. Egan, Patricia Greninger, Ultan McDermott, Mathew J. Garnett, Roger L. Jenkins, Kimberly M. Rieger-Christ, Travis B. Sullivan, Aram Francis Hezel, Andrew S. Liss, Yusuke Mizukami, Lipika Goyal, Cristina Rosa Ferrone, Andrew Xiu-Xuan Zhu, Jae Keith Joung, Kevan Michael Shokat, Cyril Henri Benes, Nabeel M. Bardeesy; Show fewer authors
Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma.
Cancer Discov. 6:727-739(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31077409; DOI=10.1002/hep.30704
Gunya Sittithumcharee, Orawan Suppramote, Kulthida Vaeteewoottacharn, Chumphon Sirisuksakun, Supawan Jamnongsong, Phatthamon Laphanuwat, Monthira Suntiparpluacha, Arriya Matha, Porncheera Chusorn, Pongsakorn Buraphat, Chumpot Kakanaporn ...Show all 21 authors... , Komgrid Charngkaew, Atit Silsirivanit, Krittiya Korphaisarn, Somchai Limsrichamrern, Pinpat Tripatara, Chawalit Pairojkul, Sopit Wongkham, Somponnat Sampattavanich, Seiji Okada, Siwanon Jirawatnotai; Show fewer authors
Dependency of cholangiocarcinoma on cyclin D-dependent kinase activity.
Hepatology 70:1614-1630(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2; PMCID=PMC6687785
Katharine Yu, Bin Chen, Dvir Aran, Joe Charalel, Christina Yau, Douglas M. Wolf, Laura J. van 't Veer, Atul Janardhan Butte, Ted Goldstein, Marina Sirota;
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat. Commun. 10:3574.1-3574.11(2019)

PubMed=32899426; DOI=10.3390/cancers12092510; PMCID=PMC7565451
Dominique Scherer, Marcela Davila-Lopez, Benjamin Goeppert, Sanna Abrahamsson, Rosa Gonzalez Silos, Igor Nova, Katherine Marcelain, Juan Carlos Roa, David Ibberson, Sinan U. Umu, Trine Ballestad Rounge ...Show all 13 authors... , Stephanie Roessler, Justo Lorenzo-Bermejo; Show fewer authors
RNA sequencing of hepatobiliary cancer cell lines: data and applications to mutational and transcriptomic profiling.
Cancers (Basel) 12:2510.1-2510.14(2020)

PubMed=35640676; DOI=10.1016/j.ajpath.2022.05.007; PMCID=PMC9472155
Abdulkadir Isidan, Ali Yenigun, Daiki Soma, Eric Aksu, Kevin Lopez, Yujin Park, Arthur Cross-Najafi, Ping Li, Debjyoti Kundu, Michael G. House, Sanjukta Chakraborty ...Show all 17 authors... , Shannon Glaser, Lindsey Kennedy, Heather Francis, Wen-Jun Zhang, Gianfranco D. Alpini, Burcin Ekser; Show fewer authors
Development and characterization of human primary cholangiocarcinoma cell lines.
Am. J. Pathol. 192:1200-1217(2022)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=39026794; DOI=10.1101/2024.07.04.601970; PMCID=PMC11257448
Vindhya Vijay, Negin Karisani, Lei Shi, Yu-Han Hung, Phuong Vu, Prabhat Kattel, Lauren Kenney, Joshua Merritt, Ramzi Adil, Qi-Biao Wu, Yuan-Li Zhen ...Show all 39 authors... , Robert Morris, Johannes Kreuzer, Meena Kathiresan, Xcanda Ixchel Herrera Lopez, Haley Ellis, Ilaria G. Gritti, Lilian Lecorgne, Ines Farag, Alexandra Popa, William Shen, Hiroyuki Kato, Qin Xu, Eranga Roshan Balasooriya, Meng-Ju Wu, Saireudee Chaturantabut, Robin Kate Kelley, James Mark Cleary, Michael Scott Lawrence, David E. Root, Cyril Henri Benes, Vikram Deshpande, Dejan Juric, William Raj Sellers, Cristina Rosa Ferrone, Wilhelm Haas, Francisca Vazquez, Gad Getz, Nabeel M. Bardeesy; Show fewer authors
Generation of a biliary tract cancer cell line atlas reveals molecular subtypes and therapeutic targets.
bioRxiv 2024:07.04.601970-07.04.601970(2024)

Cross-references
Cell line collections (Providers) CLS; 300469
JCRB; JCRB0425
RCB; RCB1960
TKG; TKG 0389
Cell line databases/resources CLO; CLO_0050009
MCCL; MCC:0000217
cancercelllines; CVCL_0324
Cell_Model_Passport; SIDM00587
Cosmic-CLP; 907069
DepMap; ACH-000976
LINCS_LDP; LCL-1788
Anatomy/cell type resources BTO; BTO_0003911
Biological sample resources BioSample; SAMN01821687
BioSample; SAMN03470954
BioSample; SAMN03471627
BioSample; SAMN10987837
Chemistry resources ChEMBL-Cells; CHEMBL3307409
ChEMBL-Targets; CHEMBL614581
GDSC; 907069
PharmacoDB; HuCCT1_635_2019
PubChem_Cell_line; CVCL_0324
Encyclopedic resources Wikidata; Q54896725
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
FANTOM5_SSTAR; 10432-106D9
GEO; GSM827224
GEO; GSM887144
GEO; GSM888216
GEO; GSM1669917
Polymorphism and mutation databases Cosmic; 907069
Cosmic; 1122350
Cosmic; 1571782
Cosmic; 2009540
Cosmic; 2629288
Cosmic; 2674063
IARC_TP53; 21387
LiGeA; CCLE_833
Progenetix; CVCL_0324
Proteomic databases PRIDE; PXD030304
PRIDE; PXD033685
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number43