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Cellosaurus HT22 (CVCL_0321)

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Cell line name HT22
Synonyms HT-22
Accession CVCL_0321
Resource Identification Initiative To cite this cell line use: HT22 (RRID:CVCL_0321)
Comments Characteristics: Highly sensitive to glutamate and is therefore used to study glutamate-induced toxicity in neuronal cells (Millipore=SCC129).
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Omics: Genomics; Chromatin accessibility; ATAC-seq.
Omics: Genomics; Chromosome conformation analysis; Hi-C.
Omics: Genomics; Whole genome sequencing.
Omics: Proteomics; PTM; Phosphorylation; Quantitative.
Omics: Proteomics; Subcellular; Secretome.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Brain, hippocampus; UBERON=UBERON_0002421.
Cell type: Neuron; CL=CL_0000540.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_U378 (HT4 [Mouse neurons])
Children:
CVCL_C0DX (HT-22 rho-0)CVCL_C0NE (HT22 Klf13 KO)CVCL_C0NF (HT22 Klf9 KO)
CVCL_C0UA (HT22 TR/TO-rKlf9 clone 2.1)CVCL_C0UB (HT22 TR/TO-mKlf13)CVCL_C0U8 (HT22-BLRP-hTRb/pSV40-BirAV5)
CVCL_C0U9 (HT22-FLBIO-rKLF9/SV40-BirAV5)CVCL_C0U7 (HT22-pSV40-BirAV5)CVCL_F1QQ (HyCyte HT22 KO-mCpt1c)
CVCL_D6BT (HyCyte HT22 KO-mNlrp3)CVCL_E2Q5 (HyCyte HT22 mSirt2_p.S368D_c.1102T>G_c.1103C>A)CVCL_D9WN (Ubigene HT22 Atg5 KO)
CVCL_D9WP (Ubigene HT22 Atp7b KO)CVCL_D9WQ (Ubigene HT22 Cbs KO)CVCL_D9WR (Ubigene HT22 Dgcr8 KO)
CVCL_D9WS (Ubigene HT22 Lox KO)CVCL_D9WT (Ubigene HT22 Prlr KO)
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): Millipore=SCC129

Markers:
Mouse STR 1-110,11
Mouse STR 1-213,17
Mouse STR 2-19
Mouse STR 3-214,15
Mouse STR 4-218.3,19.3,20.3
Mouse STR 5-513,14,15
Mouse STR 6-415.3
Mouse STR 6-712
Mouse STR 7-129
Mouse STR 8-115
Mouse STR 11-215,17,18
Mouse STR 12-120
Mouse STR 13-116.2
Mouse STR 15-320.3
Mouse STR 17-212,13,14
Mouse STR 18-317,19
Mouse STR 19-211,12
Mouse STR X-125

Run an STR similarity search on this cell line
Web pages Info; -; -; https://github.com/TNTurnerLab/HT22_genome_epigenome_functional_genomics_project
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-569.html
Provider; Salk; T09031; https://web.archive.org/web/20180601190237/http://salkinnovations.testtechnologypublisher.com:80/technology/7060
Publications

PubMed=7953717; DOI=10.1016/0006-8993(94)90334-4
John B. Davis, Pamela Maher;
Protein kinase C activation inhibits glutamate-induced cytotoxicity in a neuronal cell line.
Brain Res. 652:169-173(1994)

PubMed=9292733; DOI=10.1016/S0896-6273(00)80953-8
Yong-Hong Li, Pamela Maher, David Schubert;
A role for 12-lipoxygenase in nerve cell death caused by glutathione depletion.
Neuron 19:453-463(1997)

PubMed=19135458; DOI=10.1016/j.lfs.2008.12.008
Jun Liu, Long-Xuan Li, William Z. Suo;
HT22 hippocampal neuronal cell line possesses functional cholinergic properties.
Life Sci. 84:267-271(2009)

PubMed=26227174; DOI=10.1002/pmic.201400623
Jong-Min Woo, Do-Hyun Han, Joonho Park, Sang Jeong Kim, Youngsoo Kim;
In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.
Proteomics 15:3617-3622(2015)

PubMed=33430126; DOI=10.3390/nu13010179; PMCID=PMC7826960
Luke N. Erber, Ang Luo, Yao Gong, Montana Beeson, Mao-Lin Tu, Phu V. Tran, Yue Chen;
Iron deficiency reprograms phosphorylation signaling and reduces O-GlcNAc pathways in neuronal cells.
Nutrients 13:179.1-179.19(2021)

Cross-references
Cell line collections (Providers) CLS; 305158
Millipore; SCC129
Ubigene; YC-A004
Cell line databases/resources MCCL; MCC:0000214
Lonza; 248
Anatomy/cell type resources BTO; BTO_0003037
Chemistry resources ChEMBL-Cells; CHEMBL3307590
ChEMBL-Targets; CHEMBL614316
PubChem_Cell_line; CVCL_0321
Encyclopedic resources Wikidata; Q54896579
Proteomic databases PRIDE; PXD001597
PRIDE; PXD023294
Entry history
Entry creation04-Apr-2012
Last entry update27-Nov-2025
Version number28