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Cellosaurus HT22-BLRP-hTRb/pSV40-BirAV5 (CVCL_C0U8)

[Text version]
Cell line name HT22-BLRP-hTRb/pSV40-BirAV5
Accession CVCL_C0U8
Resource Identification Initiative To cite this cell line use: HT22-BLRP-hTRb/pSV40-BirAV5 (RRID:CVCL_C0U8)
Comments Characteristics: Expresses human THRB fused to a biotin ligase recognition peptide (BLRP).
Characteristics: Expresses C-terminal V5-tagged birA under the control of the human EF1a promoter.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11799; THRB (Note=With a N-terminal BLRP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P06709; Escherichia coli birA (Note=With a C-terminal V5 tag).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Derived from site: In situ; Brain, hippocampus; UBERON=UBERON_0002421.
Cell type: Neuron; CL=CL_0000540.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_0321 (HT22)
Category Transformed cell line
Cross-references
Cell line collections (Providers) Kerafast; EMU043
Encyclopedic resources Wikidata; Q112929762
Entry history
Entry creation23-Jun-2022
Last entry update10-Apr-2025
Version number9