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Cellosaurus HL-1 (CVCL_0303)

[Text version]
Cell line name HL-1
Synonyms HL1; HL-1 F2 P76
Accession CVCL_0303
Resource Identification Initiative To cite this cell line use: HL-1 (RRID:CVCL_0303)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12197.
Characteristics: Spontaneously contract while maintaining a differentiated cardiac phenotype (Millipore=SCC065).
Characteristics: From transgenic mice expressing a fusion gene comprised of the atrial natriuretic factor promoter and the SV40 early region.
Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Caution: The STR profile of HL-1 as distributed from Ubigene shows that the cell line that what they distribute is not HL-1 but C2C12 (Cellosaurus=CVCL_0188) or a derivative of that cell line (Ubigene=YC-C100).
Derived from site: In situ; Heart, left atrium; UBERON=UBERON_0002079.
Cell type: Cardiomyocyte; CL=CL_0002495.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3HeB/FeJ transgenic.
Hierarchy Children:
CVCL_D9WA (Ubigene HL-1 Sirt5 KO)
Originate from same individual CVCL_JK52 ! AT-1 [Mouse cardiomyocyte]
Sex of cell Female
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): Millipore=SCC065

Markers:
Mouse STR 1-117
Mouse STR 1-219
Mouse STR 2-116
Mouse STR 3-214
Mouse STR 4-220.3
Mouse STR 5-517,18
Mouse STR 6-418
Mouse STR 6-712,17
Mouse STR 7-126.2
Mouse STR 8-116
Mouse STR 11-216
Mouse STR 12-116,17
Mouse STR 13-117
Mouse STR 15-321.3
Mouse STR 17-215
Mouse STR 18-316
Mouse STR 19-213
Mouse STR X-128

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-549.html
Publications

PubMed=9501201; DOI=10.1073/pnas.95.6.2979; PMCID=PMC19680
William Creighton Claycomb, Nicholas A. Lanson Jr., Beverly S. Stallworth, Daniel B. Egeland, Joseph B. Delcarpio, Anthony Bahinski, Nicholas J. Izzo Jr.;
HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte.
Proc. Natl. Acad. Sci. U.S.A. 95:2979-2984(1998)

PubMed=10908350; DOI=10.1093/nar/28.15.2882; PMCID=PMC102680
Nicholas A. Lanson Jr., Daniel B. Egeland, Brenda A. Royals, William Creighton Claycomb;
The MRE11-NBS1-RAD50 pathway is perturbed in SV40 large T antigen-immortalized AT-1, AT-2 and HL-1 cardiomyocytes.
Nucleic Acids Res. 28:2882-2892(2000)

Patent=US6316207
William Creighton Claycomb;
Mouse cardiac muscle cell line, HL-1.
Patent number US6316207, 13-Nov-2001

PubMed=14766671; DOI=10.1152/ajpheart.00986.2003
Steven M. White, Phillip E. Constantin, William Creighton Claycomb;
Cardiac physiology at the cellular level: use of cultured HL-1 cardiomyocytes for studies of cardiac muscle cell structure and function.
Am. J. Physiol. Heart Circ. Physiol. 286:H823-H829(2004)

Cross-references
Cell line collections (Providers) Millipore; SCC065
Ubigene; YC-C100
Cell line databases/resources MCCL; MCC:0000194
Lonza; 740
Anatomy/cell type resources BTO; BTO_0003264
Encyclopedic resources Wikidata; Q29838237
Experimental variables resources EFO; EFO_0022835
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number25