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Cellosaurus C3H/10T1/2 clone 8 (CVCL_0190)

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Cell line name C3H/10T1/2 clone 8
Synonyms C3H/10T1/2-clone8; C3H/10T1/2 CL8; C3H10T1/2 clone8; C3H10T1/2CL8; 10T1/2(clone8); 10T1/2; C3H10T1-2; C3H10T1/2; C3H-10T1/2; C3H 10T1/2; C3H/10T1/2
Accession CVCL_0190
Resource Identification Initiative To cite this cell line use: C3H/10T1/2 clone 8 (RRID:CVCL_0190)
Comments Group: Space-flown cell line (cellonaut).
Part of: ENCODE project mouse cell lines.
Doubling time: 15.5 hours (Note=At 9th passage) (PubMed=4357355).
Omics: Variations; SNP array analysis.
Misspelling: C3HIOT 1/2; NCI-DTP=C3HIOT 1/2.
Misspelling: C3HT101/2; Note=Occasionally.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H.
Hierarchy Children:
CVCL_0117 (30A-5)CVCL_HA77 (7SA)CVCL_E9X8 (C3H10T1/2 clone 82A)
CVCL_E9D4 (C3H10T1/2 Con clone A1)CVCL_E9D5 (C3H10T1/2 Con clone H6)CVCL_C4L8 (C3H10T1/2 MRF4-#7)
CVCL_C4L9 (C3H10T1/2 MyoD1-#8)CVCL_C4LA (C3H10T1/2 myogenin-#1)CVCL_E9D6 (C3H10T1/2 Myc clone A7)
CVCL_E9D7 (C3H10T1/2 Myc clone C2)CVCL_E9DA (C3H10T1/2 R+M clone E5)CVCL_E9DB (C3H10T1/2 R+M clone E6)
CVCL_E9D8 (C3H10T1/2 Ras clone C4)CVCL_E9D9 (C3H10T1/2 Ras clone C10)CVCL_5I32 (C3H/10T1/2-mRuby clone 2)
CVCL_4720 (C3H/MCA clone 15)CVCL_4721 (C3H/MCA clone 16)CVCL_Y467 (C3H/MCA-58)
CVCL_C5IW (C3RL4)
Sex of cell Sex unspecified
Age at sampling Embryo
Category Spontaneously immortalized cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-279.html
Publications

PubMed=4357355
Catherine Armstrong Reznikoff, David William Brankow, Charles Heidelberger;
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
Cancer Res. 33:3231-3238(1973)

PubMed=4796800
Catherine Armstrong Reznikoff, John S. Bertram, David William Brankow, Charles Heidelberger;
Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division.
Cancer Res. 33:3239-3249(1973)

PubMed=3152871; DOI=10.1007/BF01128016
Nelwyn T. Christie, Pramila Sen, Max Costa;
Chromosomal alterations in cell lines derived from mouse rhabdomyosarcomas induced by crystalline nickel sulfide.
Biol. Met. 1:43-50(1988)

PubMed=2594967; DOI=10.2307/3577796
Mieko Yasukawa, Toyozo Terasima, Masatoshi Seki;
Radiation-induced neoplastic transformation of C3H10T1/2 cells is suppressed by ascorbic acid.
Radiat. Res. 120:456-467(1989)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
John Paul Didion, Ryan J. Buus, Zohreh Naghashfar, David W. Threadgill, Herbert Carpenter Morse 3rd, Fernando Pardo-Manuel de Villena;
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

Cross-references
Cell line collections (Providers) AddexBio; P0011003/4921
ATCC; CCL-226
CLS; 305164
ECACC; 99072801
JCRB; IFO50415
JCRB; JCRB0003
JCRB; JCRB0602 - Discontinued
JCRB; JCRB9080
KCB; KCB 2010243YJ
KCLB; 10226
NCI-DTP; C3HIOT 1/2
RCB; RCB0247
Cell line databases/resources CLO; CLO_0002120
CLO; CLO_0050546
MCCL; MCC:0000081
CLDB; cl567
CLDB; cl568
CCRID; 1101MOU-PUMC000665
CCRID; 3101MOUGNM19
CCRID; 3101MOUSCSP506
Lonza; 1346
TOKU-E; 3959
Anatomy/cell type resources BTO; BTO_0004058
BTO; BTO_0005930
Biological sample resources ENCODE; ENCBS034ENC
ENCODE; ENCBS035ENC
Chemistry resources ChEMBL-Cells; CHEMBL3307493
ChEMBL-Targets; CHEMBL614531
PubChem_Cell_line; CVCL_0190
Encyclopedic resources Wikidata; Q54808159
Experimental variables resources EFO; EFO_0002816
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number35