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Cellosaurus 2fTGH (CVCL_0115)

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Cell line name 2fTGH
Synonyms 2FTGH; 2-fTGH
Accession CVCL_0115
Resource Identification Initiative To cite this cell line use: 2fTGH (RRID:CVCL_0115)
Comments Population: Caucasian.
Characteristics: 2fTGH was derived from a HPRT negative subline of HT-1080. Expression of gpt is under the control of a IFN-alpha inducible IFI6 (6-16) promoter and is thus only expressed when IFN-alpha is present in the growth medium.
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Genetic integration: Method=Transfection; Gene=UniProtKB; P0A9M5; Escherichia coli gpt.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0317 (HT-1080)
Children:
CVCL_A3FC (2fTGH-U1A)CVCL_M019 (2fTGH-U2A)CVCL_9469 (2fTGH-U3A)
CVCL_9470 (2fTGH-U4A)CVCL_D314 (2fTGH-U4C)CVCL_D315 (2fTGH-U5A)
CVCL_D316 (2fTGH-U6A)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
STR profile Source(s): ECACC=12021508

Markers:
AmelogeninX,Y
CSF1PO12
D5S81811,13
D7S8209,10
D13S31712,14
D16S5399,12
TH016
TPOX8
vWA14,19

Run an STR similarity search on this cell line
Publications

PubMed=2513475; DOI=10.1128/mcb.9.11.4605-4612.1989; PMCID=PMC363606
Sandra Pellegrini, Joseph John, Moira Shearer, Ian Macpherson Kerr, George Robert Stark;
Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.
Mol. Cell. Biol. 9:4605-4612(1989)

PubMed=1837150; DOI=10.1073/pnas.88.24.11455; PMCID=PMC53154
Roslyn McKendry, Joseph John, David M. Flavell, Mathias Muller, Ian Macpherson Kerr, George Robert Stark;
High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.
Proc. Natl. Acad. Sci. U.S.A. 88:11455-11459(1991)

PubMed=1906577; DOI=10.1128/mcb.11.8.4189-4195.1991; PMCID=PMC361241
Joseph John, Roslyn McKendry, Sandra Pellegrini, David M. Flavell, Ian Macpherson Kerr, George Robert Stark;
Isolation and characterization of a new mutant human cell line unresponsive to alpha and beta interferons.
Mol. Cell. Biol. 11:4189-4195(1991)

Cross-references
Cell line collections (Providers) CancerTools; 151484
ECACC; 12021508 - Discontinued
Ximbio; 151484
Cell line databases/resources MCCL; MCC:0000007
cancercelllines; CVCL_0115
Anatomy/cell type resources BTO; BTO_0005765
Encyclopedic resources Wikidata; Q54584472
Experimental variables resources EFO; EFO_0022432
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number26