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Cellosaurus 2fTGH-U2A (CVCL_M019)

[Text version]
Cell line name 2fTGH-U2A
Synonyms U2A; U2
Accession CVCL_M019
Resource Identification Initiative To cite this cell line use: 2fTGH-U2A (RRID:CVCL_M019)
Comments Population: Caucasian.
Characteristics: Unresponsive to alpha and beta interferons. Partially defective in response to interferon-gamma (PubMed=1906577).
Characteristics: 2fTGH was derived from a HPRT negative subline of HT-1080. Expression of gpt is under the control of a IFN-alpha inducible IFI6 (6-16) promoter and is thus only expressed when IFN-alpha is present in the growth medium.
Transfected with: UniProtKB; P0A9M5; Escherichia coli gpt.
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph).
Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0115 (2fTGH)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
STR profile Source(s): ECACC


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PubMed=1837150; DOI=10.1073/pnas.88.24.11455
McKendry R., John J., Flavell D.M., Muller M., Kerr I.M., Stark G.R.
High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.
Proc. Natl. Acad. Sci. U.S.A. 88:11455-11459(1991)

PubMed=1906577; DOI=10.1128/mcb.11.8.4189-4195.1991
John J., McKendry R., Pellegrini S., Flavell D.M., Kerr I.M., Stark G.R.
Isolation and characterization of a new mutant human cell line unresponsive to alpha and beta interferons.
Mol. Cell. Biol. 11:4189-4195(1991)

Cell line collections (Providers) CancerTools; 151807
ECACC; 12021502
Ximbio; 151807
Encyclopedic resources Wikidata; Q54584473
Entry history
Entry creation06-May-2013
Last entry update29-Jun-2023
Version number20