| Abstract |
Two vestibular schwannoma cell lines, HEI-193 and HEI-182, as well as one
vestibular nerve schwann cell line HEI-286, were established using
retroviral-mediated transfer of the HPV E6-E7 genes. HEI-193 cells, have a
unique splice site mutation of the NF2 gene, while the NF2 gene in HEI-182
cells does not appear to be mutated. HEI-286 cells are expected to have
the wildtype NF2 gene. Both immunocytochemistry and molecular biology
techniques were used to demonstrate that these cell line are of Schwann
cell origin. Comparison of the primary tumor with HEI-193 revealed the
same NF2 mutation and an identical pattern of allele loss at multiple
loci, indicating that the established cell line had maintained many of the
properties of the original tumor. The immortalized cell lines were
nontumongemc in severe combined immunodeficient (SCID) mice and/or nude
mice, but did show altered growth properties such as higher proliferation
rate and independence of Schwann cell growth factors. As part of our
analysis we also performed microarray analysis of gene expression in the
cell lines as well as in tumors, including the original' 193 tumor. The
results obtained to date will help in our future studies aimed at
elucidating the molecular pathogenesis of NF2.
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