| Publication number |
CLPUB00790 |
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| Authors |
Kenwrick S.J., Creighton A.M. |
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| Title |
Isolation and characterization of a subline of CHO cells with induced resistance to ICRF 159.", |
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| Citation |
Br. J. Cancer 46:504-505(1982) |
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| Web pages |
https://doi.org/10.1038/bjc.1982.227 |
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| Abstract |
A subline of CHO cells has been derived, with induced resistance to the
antitumour drug ICRF 159. This subline (CHO/159-1) has a consistent
resistance index of 300 relative to the parent line in colony-forming
assays, and has the same diploid chromosome number and growth rate. Since
the presence of the detergent Tween 80 does not restore any sensitivity,
it seems unlikely that the resistance is due to a membrane alteration
affecting the uptake of the drug. No cross resistance has been
demonstrated to a variety of cytotoxIC drugs including alkylating agents
and antimetabolites. A 2-fold increase in resistance has been observed to
the anthracycline antibiotics, adriamycin and daunomycin similar to that
found earlier with 1 out of 3 BHK lines with induced resistance to ICRF
159. Although complete crossresistance is shown to ICRF 202 (a more potent
homologue of ICRF 159) CHO/159-1 is not significantly resistant to the
Chinese drug AT-1727 (the bis-N-morpholinomethyl derivative of ICRF 154).
The latter result is a little surprising since the morphological effects
of AT-1727 on cells closely resemble those produced by ICRF 159. Relevant
markers have been introduced into both the parent and the drug-resistant
sub-line and interspecific hybrids with mouse L/TK-cells have been shown
to express a codominant resistant phenotype. Analysis of the DNA content
by flow cytometry and of the chromosome number and type confirmed the
hybrid nature of these cells. The codominant nature of the resistance
makes it feasible to attempt its transfer into L/TK-cells with chromosome
or DNA preparations.
|
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| Cell lines |
CVCL_E7EP; CHO/159-1 |
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