| Abstract |
An intestinal cell line from rainbow trout, Oncorhynchus mykiss, was
developed and challenged against several bioactive components. Primary
cultures initiated from the distal segment produced the cell line, RTgutGC.
RTgutGC showed optimal growth in L15 supplemented with 10-20% fetal bovine
serum (FBS) at room temperature. RTgutGC has undergone over 100 passages
and stained minimally for beta-galactosidase, suggesting this to be an
immortal cell line. Late passage cultures gave a consistent polygonal
morphology with distinct borders. RTgutGC stained positive for alkaline
phosphatase (AP) under certain culture conditions, hence may produce
intestinal-specific alkaline phosphatase (IAP). Lipopolysaccharide (LPS)
was used as a model microbial endotoxin for determining the sensitivity of
the cells to a natural ligand in the gastrointestinal tract (GIT).
Exposure of LPS was compared between RTgutGC and two mammalian intestinal
cell lines (HT-29 and Caco-2).
|
|---|
