| Publication number |
CLPUB00663 |
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| Authors |
Leckett B. |
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| Title |
The selection, isolation, and characterization of a Chinese hamster lung fibroblast cell line resistant to an insulin-diphteria A-chain toxic conjugate molecule. |
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| Citation |
Thesis PhD (1992), McGill University Montreal, Canada |
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| Web pages |
https://escholarship.mcgill.ca/concern/theses/wp988k77t |
|---|
| Abstract |
The objective of this study was to determine how alterations in the
insulin-receptor trafficking pathway affect ligand-dependent cellular
action in Chinese hamster lung fibroblasts. Cell lines altered in their
trafficking pathway were selected using a toxic insulin-diphtheria A-chain
molecule (DTaI) which specifically interacts with the insulin receptor.
One cell variant (IV-Al-j) which exhibited decreased insulin binding and
enhanced insulin degradation did not demonstrate insulin or IGF-1
dependent cell proliferation. IV-Al-j cells also failed to produce an
insulin-stimulated tyrosine phosphorylated protein (pp175). IGF-1 binding,
insulin-stimulated hexose uptake, epidermal growth factor and thrombin
stimulated cell proliferation were unaltered in IV-Al-j cells. These
results suggest that the insulin and IGF-1 receptor may share a common
pathway post-ligand binding for cell proliferation which does not involve
EGF or thrombin, and that enhanced insulin degradation and loss of pp175
may be responsible for the loss of insulin-stimulated cell proliferation.
|
|---|
| Cell lines |
CVCL_B3UN; IV-A1-j |
|---|
