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Cellosaurus publication CLPUB00520

Publication number CLPUB00520
Authors Takebayashi S.-I.
Title Experimental studies on the heterologous transplantation of two kinds of cultured human osteogenic sarcoma cells into mice and rats.
Citation Kanazawa Daigaku Juzen Igakkai Zasshi 85:135-153(1976)
Web pages https://kanazawa-u.repo.nii.ac.jp/?action=repository_uri&item_id=18967&file_id=26&file_no=1
Abstract Morphological studies on cultured human osteogenic sarcoma cells i.e. OST cells and OST-II cells, were performed in vitro and in vivo. Not only histological and proliferating changes of OST cells and OST-II cells, but also colony formation, chromosomal and cytokinetic analyses of OST-II cells were studied in vitro. OST-II cells were heterotransplanted into the femurs of mice treated with anti-thymocyte serum. Tumor formation was observed a week after the transplantation and 9-10 weeks later huge tumors were formed on the transplanted sites, leading to the death of the mice. This fact showed that OST-II cells had a malignant character. Both OST cells and OST-II cells were heterotransplanted into femurs of rats treated with anti-thymocyte serum. The tumors grew fingertip-sized till the end of 5 weeks after transplantations, but then these tumors began to regress, and therefore the rats did not die. After both cells were transplanted into newborn rats intravenously, tumors were observed in their lungs, and these rats died 30-40 days after transplantations. In several rats, in which pulmonary tumors had been observed, tumors were histologically recognized in kidneys, adrenals and brains. In tumor tissues produced by transplantations of OST cells into femurs of rats, tumorous osteoid formation were recognized, but not recognized in the case of OST-II cells. In pulmonary tumors of both cells, osteoid tissues were not recognized. These tumors which originated in OST cells and OST-II cells histologically showed alveolar pattern, but by silver impregnation it was revealed that these tumors were nonepithelial.
Cell lines CVCL_8399; OST