| Abstract |
Morphological studies on cultured human osteogenic sarcoma cells i.e.
OST cells and OST-II cells, were performed in vitro and in vivo. Not only
histological and proliferating changes of OST cells and OST-II cells, but
also colony formation, chromosomal and cytokinetic analyses of OST-II
cells were studied in vitro. OST-II cells were heterotransplanted into the
femurs of mice treated with anti-thymocyte serum. Tumor formation was
observed a week after the transplantation and 9-10 weeks later huge
tumors were formed on the transplanted sites, leading to the death of the
mice. This fact showed that OST-II cells had a malignant character. Both
OST cells and OST-II cells were heterotransplanted into femurs of rats
treated with anti-thymocyte serum. The tumors grew fingertip-sized till
the end of 5 weeks after transplantations, but then these tumors began to
regress, and therefore the rats did not die. After both cells were
transplanted into newborn rats intravenously, tumors were observed in
their lungs, and these rats died 30-40 days after transplantations. In
several rats, in which pulmonary tumors had been observed, tumors were
histologically recognized in kidneys, adrenals and brains. In tumor
tissues produced by transplantations of OST cells into femurs of rats,
tumorous osteoid formation were recognized, but not recognized in the case
of OST-II cells. In pulmonary tumors of both cells, osteoid tissues were
not recognized. These tumors which originated in OST cells and OST-II
cells histologically showed alveolar pattern, but by silver impregnation
it was revealed that these tumors were nonepithelial.
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