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Cellosaurus publication CLPUB00273

Title Hormonal modulation of the growth of a new transplantable prostatic cell line in athymic nude mice.
Citation Urol. Res. 14:156.P8-156.P8(1986)
Abstract Multiple models in vitro and in vivo of human prostatic cancer are required for the development and preliminary testing of potential agents for the treatment of this disease. A few human prostatic tumour cell lines have been established in cell culture or as xenografts in athymic nude mice, mainly from metastatic prostate tissue. A new human prostatic cell line, TEN/12, from a primary tumour, has been serially passaged in athymic nude mice which should prove to be a useful additional model. The tumour displays both anaplastic and glandular regions, pleomorphic nuclei and abundant mitoses (65/1000 cells). Heterogenous distribution of prostatic acid phosphatase and prostate specific antigen expression is seen in sections of the xenografts. Extensive vascularization of the primary tumour and xenografts has prompted the search for angiogenesis factor expression in these cells. The TEN/12 xenografts appear to be androgen sensitive as excellent growth is observed in intact males and testosterone supplemented males but no tumours have yet been observed in females or castrated males. Analysis of nuclear steroid hormone receptor indicated high affinity binding with a Kd 4.1 x 10-9 M/l and a receptor content of 611 fmoles/mg DNA. Various hormonal modalities are presently being tested using this in vivo model. The cells from the xenografts can be cultured in semi-solid agar containing medium DMEM, supplemented with 10% foetal calf serum, insulin and 5-alpha-dihydrotestosterone, so providing an in vitro experimental model of prostatic carcinoma.
Cell lines CVCL_4765; Ten12