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Cellosaurus publication CLPUB00260

Publication number CLPUB00260
Authors Yamazaki K.
Title Tumorigenicity of established human glioma cell lines in lasat and nude mice.
Citation Neuropathology 3:29-38(1982)
Abstract Establishment of cell lines and tumorigenicity of human glioma cells were investigated from the viewpoint of morphological differentiation and anaplasia. For tissue culture, 82 human brain tumors including.34 gliomas were used, and 3 glioma cell lines (designated NP-1, NP-2, NP-3) were established. Cell line NP-1 was derived from an undifferentiated glioma out of 2, whereas NP-2 from a differentiated astrocytic glioma with mild anaplasia out of 19, NP-3 from one with marked anaplasia out of 2, and no cell line from 11 differentiated gliomas without anaplasia. Plating efficiency and colony formation in soft agar in NP-1 without S-100 and GFA proteins were higher than those in NP-2 and NP-3 with only a few GFAP staining cells. After 58 passages NP-1 (10^7 cells) began to produce tumors subcutaneously in asplenic athymic (lasat) mice in 1.5 months, and later in athymic (nude) mice in 3 to 8 months. But, after 122 passages NP-3 produced the first tumor only in lasat mouse in 4 months, and NP-2 (7.5x10^7) had no tumorigenicity in either in 8 months. The histological features were maintained by serial passaging in nude mice, and so the tumor cells of heterotransplants of NP-1 had fewer 10nm filaments and junctional complexes than those of NP-3. It was suggested that establishment of cell lines and tumorigenicity of human glioma cells were presumably related to the grade of differentiation: and anaplasia of glioma cells, and especially to differentiation rather than anaplasia. In addition, it would be extremely useful for the precise analysis of tumorigenicity of human glioma cells to use lasat and nude mice together.
Cell lines CVCL_1R15; NP-2
CVCL_1R16; NP-3