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Cellosaurus publication CLPUB00114

Publication number CLPUB00114
Authors Seman G., Hunter S.J., Dmochowski L.
Title Two cell lines (SH-2 and SH-3) derived from human breast cancer.
Citation Proc. Am. Assoc. Cancer Res. 16:59-59(1975)
Abstract A cell line (SH-2) has been established from breast tumor biopsy after 3 years in culture and another (SH-3) after 13 months in culture from pleural effusion of a breast cancer patient. Both lines from Caucasian patients have been established after spontaneous emergence of refractile, fast-growing cells. Line SH-2 differs from line SH-3 by the presence of multinucleated cells. By electron microscopy, the cells contain desmosomes, tonofibrils, are undifferentiated, and are free of mycoplasma. Chromosome complement of the cells is hypotetraploid, is different from that of HeLa cells, and contains a large metacentric chromosome. Like HeLa and MAC-21 cells, SH-2 and SH-3 cells possess only the A band of glucose phosphate dehydrogenase. Unlike our HeLa cells, cells of both lines contain the bone-type alkaline phosphatase. They release in tissue culture medium a number of human serum proteins, including IgG, alpha-2 macroglobulin and transferrin. Tissue culture medium does not contain reverse transcriptase activity. Attempts to induce virus particles synthesis with BdUr have so far been unsuccessful. Cells of both lines grow in nude mice. The cell lines are a valuable tool for virological, immunological and hormonal studies.
Cell lines CVCL_M622; SH-2 [Human contaminated breast cancer]
CVCL_M383; SH-3