| Abstract |
A novel myeloma cell line, designated NOP-1, was established from the bone
marrow of a male patient with IgA-kappa type multiple myeloma. NOP-1 cells
grow in single-cell suspension with doubling time of 36 hours. NOP-1 cells
were uniformly positive for cytoplasmic and surface immunoglobuline (Ig) of
IgA-kappa and J-chain of IgA, but were negative for other classes of Ig.
Surface marker analysis by an indirect immunofluorescent technique
revealed the presence of CD4 and PCA-1 antigen. NOP-1 cells were uniformly
negative for Ia-like antigen, B cell specific antigens such as CD9, CD19,
CD20, CD21 and CC24, T cell specific antigens except CD4, myeloid,
erythroid and megakaryocyte-platelets specific antigens. NOP-1 cells
possessed no markers for E-rosette, C3 receptor and IgG- and IgM-Fc receptor.
Epstein-Barr virus associated nuclear antigen was negative in NOP-1 cells.
Culture supernatant contained human Ig of IgA-k. Chromosome analysis of
this cell line revealed a human male hyperdiploid karyotype with a modal
chromosome number of 74 to 76. NOP-1 cells were successfully transplanted
and have been maintained into untreated normal athymic nude mice (KSN).
After injection of 5x10^7 NOP-1 cells into intraperitoneal space,
sanguineous ascites developed in 100% of injected mice. This
transplantable cell line was designated NOP-1/nu and has been maintained
over 10 passages. This novel human myeloma cell line may provide a useful
model for the study of cell lineage of multiple myeloma and of
chemotherapeutic evaluation of multiple myeloma in vitro, and in vivo.
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