| Publication number |
CLPUB00037 |
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| Authors |
Freund M., Kleine H.D., Lux E., Exeriede G., Hanauske A.-R., Herbst H., Vainchenker W., Schweigerer L., Fonatsch C., Poliwoda H. |
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| Title |
ELF-153: a novel cell line derived from a patient with acute myelofibrosis and AML. |
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| Citation |
Blut 61:128-128(1990) |
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| Abstract |
A novel GM-CSF dependent cell line derived from a patient with acute
myelofibrosis and subsequent overt AML M1 FAB has been established.
Cytochemistry of ELF-153 is undifferentiated. Surface markers are: CD33
93%, CD34 99%, CDw41 72%, CD7 46%, CDw42b 7%. Electron microscopy shows a
clear megakaryocytic component with demarkation membranes in mature cells.
Cytogenetics was identical to that of the patient and revealed a monosomy
7, 14 and 17, a deletion (5)(q13q31), and t(12;14) (p11.2;q11.2). The
supernatant of ELF-153 stimulates the growth of NRK-49F fibroblasts in a
cloning assay and the growth of bovine endothelial cells. It stimulates
HEL cells to secrete IL-6. In co-cultivation ELF-153 adheres to
fibroblasts and endothelial cells. After subcutaneous injection into nude
mice a formation of tumors with a rigid texture was observed. Histological
examination demonstrated a dense infiltration by blast cells, and a
pronounced reticular fibrosis consisting exclusively of collagen III. The
cell line ELF 153-GM will facilitate research on the interrelationship of
malignant hemopoiesis and its stroma.
|
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| Cell lines |
CVCL_2031; ELF-153 |
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