Cellosaurus cell line Hep-2044 (CVCL

Cellosaurus Hep-2044 (CVCL_ZY40)

Cross-references
Cell line name	Hep-2044
Synonyms	Hep2044
Accession	CVCL_ZY40
Resource Identification Initiative	To cite this cell line use: Hep-2044 (RRID:CVCL_ZY40)
Comments	Characteristics: The CCDN1, CDK4 and TERT genes expression can be silenced by administration of tetracycline/doxycycline through a Tet-Off system (PubMed=33060611). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1582; CCND1. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Derived from site: In situ; Liver; UBERON=UBERON_0002107. Cell type: Hepatocyte; CL=CL_0000182.
Disease	Biliary atresia (NCIt: C34421) Isolated biliary atresia (ORDO: Orphanet_30391)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell	Female
Age at sampling	5M
Category	Telomerase immortalized cell line
Publications	PubMed=33060611; DOI=10.1038/s41598-020-73992-3; PMCID=PMC7567112 Manami Nishiwaki, Masashi Toyoda, Yoshie Oishi, Seiichi Ishida, Shin-ichiro Horiuchi, Hatsune Makino-Itou, Tohru Kimura, Shin-ichi Ohno, Takashi Ohkura, Shin Enosawa, Hidenori Akutsu ...Show all 15 authors... , Atsuko Nakazawa, Mureo Kasahara, Tohru Kiyono, Akihiro Umezawa; Show fewer authors Immortalization of human hepatocytes from biliary atresia with CDK4(R24C), cyclin D1, and TERT for cytochrome P450 induction testing. Sci. Rep. 10:17503.1-17503.13(2020)
Encyclopedic resources	Wikidata; Q102114120
Entry history
Entry creation	29-Oct-2020
Last entry update	14-Aug-2025
Version number	9