ID   Hep-2044
AC   CVCL_ZY40
SY   Hep2044
DR   Wikidata; Q102114120
RX   PubMed=33060611;
CC   Characteristics: The CCDN1, CDK4 and TERT genes expression can be silenced by administration of tetracycline/doxycycline through a Tet-Off system (PubMed=33060611).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1582; CCND1.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
DI   NCIt; C34421; Biliary atresia
DI   ORDO; Orphanet_30391; Isolated biliary atresia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   5M
CA   Telomerase immortalized cell line
DT   Created: 29-10-20; Last updated: 19-12-24; Version: 8
//
RX   PubMed=33060611; DOI=10.1038/s41598-020-73992-3; PMCID=PMC7567112;
RA   Nishiwaki M., Toyoda M., Oishi Y., Ishida S., Horiuchi S.-i.,
RA   Makino-Itou H., Kimura T., Ohno S.-i., Ohkura T., Enosawa S.,
RA   Akutsu H., Nakazawa A., Kasahara M., Kiyono T., Umezawa A.;
RT   "Immortalization of human hepatocytes from biliary atresia with
RT   CDK4(R24C), cyclin D1, and TERT for cytochrome P450 induction
RT   testing.";
RL   Sci. Rep. 10:17503.1-17503.13(2020).
//