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Cellosaurus HeLa FLAG-SNAP-hTERT (CVCL_ZM01)

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Cell line name HeLa FLAG-SNAP-hTERT
Synonyms FLAG-SNAP-hTERT expressing HeLa
Accession CVCL_ZM01
Resource Identification Initiative To cite this cell line use: HeLa FLAG-SNAP-hTERT (RRID:CVCL_ZM01)
Comments Population: African American.
Characteristics: Using CRISPR/Cas9 a FLAG-SNAP-tag was fused to the N-terminus of TERT. The SNAP-tag may be used to visualize subcellular localization of the low abundant endogenous TERT protein.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly180_Asn207del and 19 mutations = SNAP-tag).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): Millipore=SCC112

Markers:
AmelogeninX
CSF1PO9,10
D3S135815,18
D5S81811,12
D7S8208,12
D8S117912
D13S31713.3
D16S5399,10
D18S5116
D21S1127
FGA18,21
Penta D8
Penta E7,17
TH017
TPOX12
vWA16,18

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Publications

PubMed=26553065; DOI=10.1186/s13059-015-0791-1; PMCID=PMC4640169
Ling-He Xi, Jens C. Schmidt, Arthur John Zaug, Dante R. Ascarrunz, Thomas Robert Cech;
A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression.
Genome Biol. 16:231.1-231.17(2015)

Cross-references
Cell line collections (Providers) Millipore; SCC112
Encyclopedic resources Wikidata; Q98126680
Entry history
Entry creation02-Jul-2020
Last entry update10-Apr-2025
Version number10