ID   HeLa FLAG-SNAP-hTERT
AC   CVCL_ZM01
SY   FLAG-SNAP-hTERT expressing HeLa
DR   Millipore; SCC112
DR   Wikidata; Q98126680
RX   PubMed=26553065;
CC   Population: African American.
CC   Characteristics: Using CRISPR/Cas9 a FLAG-SNAP-tag was fused to the N-terminus of TERT. The SNAP-tag may be used to visualize subcellular localization of the low abundant endogenous TERT protein.
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly180_Asn207del and 19 mutations = SNAP-tag).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
ST   Source(s): Millipore=SCC112
ST   Amelogenin: X
ST   CSF1PO: 9,10
ST   D13S317: 13.3
ST   D16S539: 9,10
ST   D18S51: 16
ST   D21S11: 27
ST   D3S1358: 15,18
ST   D5S818: 11,12
ST   D7S820: 8,12
ST   D8S1179: 12
ST   FGA: 18,21
ST   Penta D: 8
ST   Penta E: 7,17
ST   TH01: 7
ST   TPOX: 12
ST   vWA: 16,18
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0030 ! HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 02-07-20; Last updated: 10-04-25; Version: 10
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RX   PubMed=26553065; DOI=10.1186/s13059-015-0791-1; PMCID=PMC4640169;
RA   Xi, Ling-He
RA   Schmidt, Jens C.
RA   Zaug, Arthur John
RA   Ascarrunz, Dante R.
RA   Cech, Thomas Robert
RT   "A novel two-step genome editing strategy with CRISPR-Cas9 provides
RT   new insights into telomerase action and TERT gene expression.";
RL   Genome Biol. 16:231.1-231.17(2015).
//