Cellosaurus N27-A (CVCL_ZJ56)
| Cell line name | N27-A |
|---|---|
| Accession | CVCL_ZJ56 |
| Resource Identification Initiative | To cite this cell line use: N27-A (RRID:CVCL_ZJ56) |
| Comments | Characteristics: Improved in vitro model of dopamine neurons for research on Parkinson's disease. Expresses TH and DAT at 4 times the level of the parent cell line. Also expresses VMAT2, dopaminergic transcription factors, dopaminergic storage protein (PubMed=27512998). Doubling time: ~36 hours (PubMed=27512998). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo). Derived from site: In situ; Embryonic brain, mesencephalon; UBERON=UBERON_0009616. |
| Species of origin | Rattus norvegicus (Rat) (NCBI Taxonomy: 10116) |
| Hierarchy | Parent: CVCL_D584 (N27) |
| Sex of cell | Sex unspecified |
| Age at sampling | 12FD |
| Category | Transformed cell line |
| Publications | PubMed=27512998; DOI=10.1371/journal.pone.0160847; PMCID=PMC4981411 |
| Cross-references | |
| Cell line collections (Providers) | Millipore; SCC196 |
| Encyclopedic resources | Wikidata; Q98127400 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 14-Aug-2025 |
| Version number | 8 |