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Cellosaurus N27 (CVCL_D584)

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Cell line name N27
Synonyms N-27; 1RB3AN27; 1RB3A-N27
Accession CVCL_D584
Resource Identification Initiative To cite this cell line use: N27 (RRID:CVCL_D584)
Comments Characteristics: Used as a dopaminergic neuron model for in vitro and in vivo studies (Millipore=SCC048).
Characteristics: During many passages over 20 years, the original cell line became a mixture of cell types with less than 5% TH-positive dopamine neurons (PubMed=27512998).
Doubling time: 24 hours (PubMed=27512998).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
Derived from site: In situ; Embryonic brain, mesencephalon; UBERON=UBERON_0009616.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Hierarchy Children:
CVCL_ZJ56 (N27-A)
Sex of cell Sex unspecified
Age at sampling 12FD
Category Transformed cell line
Publications

PubMed=7820310; DOI=10.1007/BF02631258
Kedar N. Prasad, Erika Carvalho, Susan Kentroti, Judith Edwards-Prasad, Curt R. Freed, Antonia Vernadakis;
Establishment and characterization of immortalized clonal cell lines from fetal rat mesencephalic tissue.
In Vitro Cell. Dev. Biol. Anim. 30:596-603(1994)

PubMed=22863662; DOI=10.1111/j.1582-4934.2012.01607.x; PMCID=PMC4118228
Asir Alwin Prem Anand, S. Gowri Sankar, Kokila V. Vani;
Immortalization of neuronal progenitors using SV40 large T antigen and differentiation towards dopaminergic neurons.
J. Cell. Mol. Med. 16:2592-2610(2012)

PubMed=27512998; DOI=10.1371/journal.pone.0160847; PMCID=PMC4981411
Lu Gao, Wen-Bo Zhou, Breanna Symmes, Curt R. Freed;
Re-cloning the N27 dopamine cell line to improve a cell culture model of Parkinson's disease.
PLoS ONE 11:e0160847.1-e0160847.18(2016)

Cross-references
Cell line collections (Providers) Millipore; SCC048
Anatomy/cell type resources BTO; BTO_0002393
Encyclopedic resources Wikidata; Q54907338
Entry history
Entry creation22-Oct-2012
Last entry update14-Aug-2025
Version number16