Cell line name |
HepaFH3 |
Accession |
CVCL_ZG37 |
Resource Identification Initiative |
To cite this cell line use: HepaFH3 (RRID:CVCL_ZG37) |
Comments |
Characteristics: Has an extended lifespan due to the lentiviral transfer of Upcyte(R) proliferation genes. Doubling time: ~28 hours (PubMed=26715207). Derived from site: In situ; Liver; UBERON=UBERON_0002107. Cell type: Hepatocyte; CL=CL_0000182. |
Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
Sex of cell |
Female |
Age at sampling |
59Y |
Category |
Finite cell line |
Publications | PubMed=26715207; DOI=10.1002/cbin.10574 Herzog N., Hansen M., Miethbauer S., Schmidtke K.-U., Anderer U., Lupp A., Sperling S., Seehofer D., Damm G., Scheibner K., Kupper J.-H. Primary-like human hepatocytes genetically engineered to obtain proliferation competence display hepatic differentiation characteristics in monolayer and organotypical spheroid cultures. Cell Biol. Int. 40:341-353(2016) DOI=10.17169/refubium-1249 Sperling S. Genetically modified Upcyte(R) hepatocytes as a powerful alternative to primarily human hepatocytes and the hepatocellular carcinoma cell line HepG2 in the in vitro steatosis model. Thesis MD (2018); Charite - Universitatsmedizin Berlin; Berlin; Germany PubMed=30347615; DOI=10.3233/CH-189321 Kammerer S., Kupper J.-H. Optimized protocol for induction of cytochrome P450 enzymes 1A2 and 3A4 in human primary-like hepatocyte cell strain HepaFH3 to study in vitro toxicology. Clin. Hemorheol. Microcirc. 70:563-571(2018) PubMed=31561354; DOI=10.3233/CH-199226; PMCID=PMC6918903 Schulz C., Kammerer S., Kupper J.-H. NADPH-cytochrome P450 reductase expression and enzymatic activity in primary-like human hepatocytes and HepG2 cells for in vitro biotransformation studies. Clin. Hemorheol. Microcirc. 73:249-260(2019) |
Cross-references |
Encyclopedic resources |
Wikidata; Q98126683
|
Entry history |
Entry creation | 02-Jul-2020 |
Last entry update | 29-Jun-2023 |
Version number | 5 |
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