ID   HepaFH3
AC   CVCL_ZG37
DR   Wikidata; Q98126683
RX   DOI=10.17169/refubium-1249;
RX   PubMed=26715207;
RX   PubMed=30347615;
RX   PubMed=31561354;
CC   Characteristics: Has an extended lifespan due to the lentiviral transfer of Upcyte(R) proliferation genes.
CC   Doubling time: ~28 hours (PubMed=26715207).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   59Y
CA   Finite cell line
DT   Created: 02-07-20; Last updated: 29-06-23; Version: 5
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RX   DOI=10.17169/refubium-1249;
RA   Sperling S.;
RT   "Genetically modified Upcyte(R) hepatocytes as a powerful alternative to
RT   primarily human hepatocytes and the hepatocellular carcinoma cell line
RT   HepG2 in the in vitro steatosis model.";
RL   Thesis MD (2018); Charite - Universitatsmedizin Berlin; Berlin; Germany.
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RX   PubMed=26715207; DOI=10.1002/cbin.10574;
RA   Herzog N., Hansen M., Miethbauer S., Schmidtke K.-U., Anderer U.,
RA   Lupp A., Sperling S., Seehofer D., Damm G., Scheibner K.,
RA   Kupper J.-H.;
RT   "Primary-like human hepatocytes genetically engineered to obtain
RT   proliferation competence display hepatic differentiation
RT   characteristics in monolayer and organotypical spheroid cultures.";
RL   Cell Biol. Int. 40:341-353(2016).
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RX   PubMed=30347615; DOI=10.3233/CH-189321;
RA   Kammerer S., Kupper J.-H.;
RT   "Optimized protocol for induction of cytochrome P450 enzymes 1A2 and
RT   3A4 in human primary-like hepatocyte cell strain HepaFH3 to study in
RT   vitro toxicology.";
RL   Clin. Hemorheol. Microcirc. 70:563-571(2018).
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RX   PubMed=31561354; DOI=10.3233/CH-199226; PMCID=PMC6918903;
RA   Schulz C., Kammerer S., Kupper J.-H.;
RT   "NADPH-cytochrome P450 reductase expression and enzymatic activity in
RT   primary-like human hepatocytes and HepG2 cells for in vitro
RT   biotransformation studies.";
RL   Clin. Hemorheol. Microcirc. 73:249-260(2019).
//