Cellosaurus 46C-Dip2a KO (CVCL_ZE88)
| Cell line name | 46C-Dip2a KO |
|---|---|
| Synonyms | IBMSe001-A-1 |
| Accession | CVCL_ZE88 |
| Resource Identification Initiative | To cite this cell line use: 46C-Dip2a KO (RRID:CVCL_ZE88) |
| Comments | Characteristics: Using CRISPR/Cas9 exon 8 of Dip2a was replaced by a PGK-Puro-P2A-mCherry construct. Characteristics: Expresses GFP under the control of the Sox1 promoter. Knockout cell: Method=CRISPR/Cas9; MGI; MGI:2385920; Dip2a. Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola. |
| Hierarchy | Parent: CVCL_9108 (ES-E14TG2a) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=32361465; DOI=10.1016/j.scr.2020.101778 |
| Cross-references | |
| Cell line databases/resources | SKIP; SKIP005729 |
| Encyclopedic resources | Wikidata; Q98125404 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 29-Jun-2023 |
| Version number | 6 |