ID   46C-Dip2a KO
AC   CVCL_ZE88
SY   IBMSe001-A-1
DR   SKIP; SKIP005729
DR   Wikidata; Q98125404
RX   PubMed=32361465;
CC   Characteristics: Using CRISPR/Cas9 exon 8 of Dip2a was replaced by a PGKp-PuroR-P2A-mCherry construct (PubMed=32361465).
CC   Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; MGI; MGI:2385920; Dip2a.
CC   Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_Y482 ! 46C
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 02-07-20; Last updated: 19-12-24; Version: 8
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RX   PubMed=32361465; DOI=10.1016/j.scr.2020.101778;
RA   Yao M.-Z., Yang Q., Lian M.-Q., Su P.-F., Cui X.-G., Ren T.-L.,
RA   Wang X.-D., Wu C.-X., Zheng Y.-W.;
RT   "Generation of Dip2a homozygous knockout murine ES cell line
RT   IBMSe001-A-1 via CRISPR/Cas9 technology.";
RL   Stem Cell Res. 45:101778-101778(2020).
//