ID   46C-Dip2a KO
AC   CVCL_ZE88
SY   IBMSe001-A-1
DR   SKIP; SKIP005729
DR   Wikidata; Q98125404
RX   PubMed=32361465;
CC   Characteristics: Using CRISPR/Cas9 exon 8 of Dip2a was replaced by a PGK-Puro-P2A-mCherry construct.
CC   Characteristics: Expresses GFP under the control of the Sox1 promoter.
CC   Knockout cell: Method=CRISPR/Cas9; MGI; MGI:2385920; Dip2a.
CC   Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_9108 ! ES-E14TG2a
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 02-07-20; Last updated: 29-06-23; Version: 6
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RX   PubMed=32361465; DOI=10.1016/j.scr.2020.101778;
RA   Yao M.-Z., Yang Q., Lian M.-Q., Su P.-F., Cui X.-G., Ren T.-L.,
RA   Wang X.-D., Wu C.-X., Zheng Y.-W.;
RT   "Generation of Dip2a homozygous knockout murine ES cell line
RT   IBMSe001-A-1 via CRISPR/Cas9 technology.";
RL   Stem Cell Res. 45:101778-101778(2020).
//