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Cellosaurus mPAM-CD163-GFP (CVCL_ZE12)

[Text version]
Cell line name mPAM-CD163-GFP
Accession CVCL_ZE12
Resource Identification Initiative To cite this cell line use: mPAM-CD163-GFP (RRID:CVCL_ZE12)
Comments Virology: Highly susceptible to infection by porcine reproductive and respiratory syndrome virus type 2 (PRRSV-2) strains JXA1 and SD1 (PubMed=32402349).
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=VGNC; VGNC:112737; Pig CD163.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
Derived from site: In situ; Lung, alveolus; UBERON=UBERON_0002299.
Cell type: Alveolar macrophage; CL=CL_0000583.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Breed/subspecies: Landrace; VBO=VBO_0001161.
Hierarchy Parent: CVCL_0F14 (IPAM 3D4/21)
Sex of cell Sex unspecified
Age at sampling 12W
Category Transformed cell line
Publications

PubMed=32402349; DOI=10.1016/j.vetmic.2020.108690
Yu-Lin Xu, Shao-Peng Wu, Yung-Gang Li, Feng-Xia Sun, Qiu-Ju Wang, Qing Zhao, Jiang Yu, Fu-Lin Tian, Jia-Qiang Wu, Rui-Liang Zhu, Jun Peng;
A porcine alveolar macrophage cell line stably expressing CD163 demonstrates virus replication and cytokine secretion characteristics similar to primary alveolar macrophages following PRRSV infection.
Vet. Microbiol. 244:108690.1-108690.6(2020)

Cross-references
Encyclopedic resources Wikidata; Q98127279
Entry history
Entry creation02-Jul-2020
Last entry update14-Aug-2025
Version number13