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Cellosaurus Cre4 rho-0#11 (CVCL_ZB95)

[Text version]
Cell line name Cre4 rho-0#11
Accession CVCL_ZB95
Resource Identification Initiative To cite this cell line use: Cre4 rho-0#11 (RRID:CVCL_ZB95)
Comments Characteristics: Cell line devoid of mitochondrial DNA (rho0). Produced by propagation for 3 weeks of the parent cell line in a medium containing 5 ug/ml EtBr.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Genetic integration: Method=Transgenic mouse; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2).
Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: B6.Cg-Tg(CAG-cre/Esr1)5Amc/J x Lig3LoxP.
Hierarchy Parent: CVCL_ZB94 (Cre4)
Sex of cell Sex unspecified
Age at sampling 13-14FD
Category Transformed cell line
Publications

PubMed=23884459; DOI=10.1074/jbc.M113.472977; PMCID=PMC3772206
Inna N. Shokolenko, Rafik Z. Fayzulin, Sachin Katyal, Peter J. McKinnon, Glenn L. Wilson, Mikhail F. Alexeyev;
Mitochondrial DNA ligase is dispensable for the viability of cultured cells but essential for mtDNA maintenance.
J. Biol. Chem. 288:26594-26605(2013)

Cross-references
Cell line collections (Providers) Kerafast; ESA108
Encyclopedic resources Wikidata; Q98125689
Entry history
Entry creation02-Jul-2020
Last entry update14-Aug-2025
Version number9