Cellosaurus WAe009-A-29 (CVCL_ZB43)
| Cell line name | WAe009-A-29 |
|---|---|
| Synonyms | H9-CAG-ChR2-EYFP; H9-AAVS-Puro-CAG-ChR2-eYFP #11CB3; WISCe010-A-29 |
| Accession | CVCL_ZB43 |
| Resource Identification Initiative | To cite this cell line use: WAe009-A-29 (RRID:CVCL_ZB43) |
| Comments | From: University of Wisconsin; Madison; USA. Registration: Swiss research registry; BAG-hES-IMP-0102. Characteristics: Using CRISPR/Cas9 a PuroR-CAG-ChR2(H134R)-eYFP construct was was introduced in the AAVS1 safe harbor locus (PubMed=31981924). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q8RUT8; Chlamydomonas reinhardtii channelrhodopsin-2 (cop4) (Note=With p.His134Arg). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_9773 (WA09) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=31981924; DOI=10.1016/j.isci.2020.100829; PMCID=PMC6993006 |
| Cross-references | |
| Cell line collections (Providers) | WiCell; h9-cag-chr2-eyfp |
| Cell line databases/resources | hPSCreg; WAe009-A-29 |
| Encyclopedic resources | Wikidata; Q98134755 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 14-Aug-2025 |
| Version number | 9 |