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Cellosaurus SfSWT-1 (CVCL_Z366)

[Text version]
Cell line name SfSWT-1
Synonyms Sf SWT1; SfSWT1; Mimic; Mimic Sf9
Accession CVCL_Z366
Resource Identification Initiative To cite this cell line use: SfSWT-1 (RRID:CVCL_Z366)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Characteristics: Has been engineered to stably express a variety of mammalian glycosyltransferases thus allowing for production of biantennary, terminally sialyated N-glycans from insect cells. Can be used to produce more mammalian-like proteins in both baculovirus and stable insect expression systems (Thermo Fisher).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7044; MGAT1.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7045; MGAT2.
Genetic integration: Method=Transfection; Gene=MGI; MGI:1316743; St3gal4.
Genetic integration: Method=Transfection; Gene=RGD; 3676; St6gal1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=VGNC; VGNC:26388; Bovine B4GALT1.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Species of origin Spodoptera frugiperda (Fall armyworm) (NCBI Taxonomy: 7108)
Hierarchy Parent: CVCL_Z364 (Sfbeta4GalT)
Children:
CVCL_Z367 (SfSWT-3)
Sex of cell Female
Age at sampling Pupal stage
Category Spontaneously immortalized cell line
Web pages Provider; Thermo Fisher; 12552014; https://tools.thermofisher.com/content/sfs/manuals/mimic_cell_man.pdf
Provider; Thermo Fisher; 12552014; https://www.thermofisher.com/order/catalog/product/12552014
Publications

PubMed=12475259; DOI=10.1021/bi026455d; PMCID=PMC3612895
Hollister J.R., Grabenhorst E., Nimtz M., Conradt H.S., Jarvis D.L.
Engineering the protein N-glycosylation pathway in insect cells for production of biantennary, complex N-glycans.
Biochemistry 41:15093-15104(2002)

PubMed=12626399; DOI=10.1093/glycob/cwg051; PMCID=PMC3612900
Aumiller J.J., Hollister J.R., Jarvis D.L.
A transgenic insect cell line engineered to produce CMP-sialic acid and sialylated glycoproteins.
Glycobiology 13:497-507(2003)

PubMed=20574041; DOI=10.1093/glycob/cwq099; PMCID=PMC2948823
Hillar A., Jarvis D.L.
Re-visiting the endogenous capacity for recombinant glycoprotein sialylation by baculovirus-infected Tn-4h and DpN1 cells.
Glycobiology 20:1323-1330(2010)

Cross-references
Encyclopedic resources Wikidata; Q54953032
Entry history
Entry creation10-Apr-2015
Last entry update10-Apr-2025
Version number16