ID   SfSWT-1
AC   CVCL_Z366
SY   Sf SWT1; SfSWT1; Mimic; Mimic Sf9
DR   Wikidata; Q54953032
RX   PubMed=12475259;
RX   PubMed=12626399;
RX   PubMed=20574041;
WW   Provider; Thermo Fisher; 12552014; https://tools.thermofisher.com/content/sfs/manuals/mimic_cell_man.pdf
WW   Provider; Thermo Fisher; 12552014; https://www.thermofisher.com/order/catalog/product/12552014
CC   Group: Insect cell line.
CC   Group: Recombinant protein production insect cell line.
CC   Characteristics: Has been engineered to stably express a variety of mammalian glycosyltransferases thus allowing for production of biantennary, terminally sialyated N-glycans from insect cells. Can be used to produce more mammalian-like proteins in both baculovirus and stable insect expression systems (Thermo Fisher).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7044; MGAT1.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7045; MGAT2.
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:1316743; St3gal4.
CC   Genetic integration: Method=Transfection; Gene=RGD; 3676; St6gal1.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=VGNC; VGNC:26388; Bovine B4GALT1.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
OX   NCBI_TaxID=7108; ! Spodoptera frugiperda (Fall armyworm)
HI   CVCL_Z364 ! Sfbeta4GalT
SX   Female
AG   Pupal stage
CA   Spontaneously immortalized cell line
DT   Created: 10-04-15; Last updated: 10-04-25; Version: 16
//
RX   PubMed=12475259; DOI=10.1021/bi026455d; PMCID=PMC3612895;
RA   Hollister J.R., Grabenhorst E., Nimtz M., Conradt H.S., Jarvis D.L.;
RT   "Engineering the protein N-glycosylation pathway in insect cells for
RT   production of biantennary, complex N-glycans.";
RL   Biochemistry 41:15093-15104(2002).
//
RX   PubMed=12626399; DOI=10.1093/glycob/cwg051; PMCID=PMC3612900;
RA   Aumiller J.J., Hollister J.R., Jarvis D.L.;
RT   "A transgenic insect cell line engineered to produce CMP-sialic acid
RT   and sialylated glycoproteins.";
RL   Glycobiology 13:497-507(2003).
//
RX   PubMed=20574041; DOI=10.1093/glycob/cwq099; PMCID=PMC2948823;
RA   Hillar A., Jarvis D.L.;
RT   "Re-visiting the endogenous capacity for recombinant glycoprotein
RT   sialylation by baculovirus-infected Tn-4h and DpN1 cells.";
RL   Glycobiology 20:1323-1330(2010).
//