Cellosaurus WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 (CVCL_YU29)
| Cell line name | WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 |
|---|---|
| Synonyms | AICS-0089-061; UCSFi001-A-39 |
| Accession | CVCL_YU29 |
| Resource Identification Initiative | To cite this cell line use: WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 (RRID:CVCL_YU29) |
| Comments | Part of: Allen Cell Collection. Population: Japanese. Characteristics: Using CRISPR/Cas9 FBL has been endogenously tagged in one allele at the C-terminus with mEGFP, NPM1 has been endogenously tagged in one allele at the C-terminus with mTagRFP-T and a dCas9-TagBFP-KRAB construct is expressed from a CAGGS promoter edited into the 2nd intron of the CLYBL safe harbor. Transfected with: UniProtKB; P42212; GFP (with p.Tyr66His and p.Tyr145Phe = BFP). Transfected with: UniProtKB; H3JQU7; Entacmaea quadricolor red fluorescent protein eqFP578 (mutated to obtain mTagRFP-T). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (deactivated version with KRAB domain; dCas9-KRAB). Derived from sampling site: Leg; skin. Cell type=Fibroblast. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_Y803 (GM25256) |
| Sex of cell | Male |
| Age at sampling | 30Y |
| Category | Induced pluripotent stem cell |
| Web pages | https://www.allencell.org/cell-catalog.html |
| Cross-references | |
| Cell line collections | Coriell; AICS-0089-061 |
| Cell line databases/resources | hPSCreg; UCSFi001-A-39 |
| Biological sample resources | BioSamples; SAMEA7655481 |
| Encyclopedic resources | Wikidata; Q98135041 |
| Entry history | |
| Entry creation | 12-Mar-2020 |
| Last entry update | 21-Mar-2023 |
| Version number | 7 |