Cellosaurus WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 (CVCL_YU29)
| Cell line name | WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 |
|---|---|
| Synonyms | AICS-0089-061; AICSi002-A-39; UCSFi001-A-39 |
| Accession | CVCL_YU29 |
| Resource Identification Initiative | To cite this cell line use: WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 (RRID:CVCL_YU29) |
| Comments | Part of: Allen Cell Collection. Population: Japanese. Characteristics: Using CRISPR/Cas9 FBL has been endogenously tagged in one allele at the C-terminus with mEGFP, NPM1 has been endogenously tagged in one allele at the C-terminus with mTagRFP-T and a dCas9-TagBFP-KRAB construct is expressed from a CAGGS promoter edited into the 2nd intron of the CLYBL safe harbor. Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; QKFJN; mEGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; LF3LJ; TagRFP-T (Note=Red fluorescent protein derived from eqFP578). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB). Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511. Cell type: Fibroblast of skin; CL=CL_0002620. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_Y803 (GM25256) |
| Sex of cell | Male |
| Age at sampling | 30Y |
| Category | Induced pluripotent stem cell |
| Web pages | Info; Allen Institute; -; https://www.allencell.org/cell-catalog.html |
| Cross-references | |
| Cell line collections (Providers) | Coriell; AICS-0089-061 |
| Cell line databases/resources | hPSCreg; UCSFi001-A-39 |
| Biological sample resources | BioSamples; SAMEA7655481 |
| Encyclopedic resources | Wikidata; Q98135041 |
| Entry history | |
| Entry creation | 12-Mar-2020 |
| Last entry update | 10-Apr-2025 |
| Version number | 12 |