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Cellosaurus WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 (CVCL_YU29)

[Text version]
Cell line name WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61
Synonyms AICS-0089-061; UCSFi001-A-39
Accession CVCL_YU29
Resource Identification Initiative To cite this cell line use: WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 (RRID:CVCL_YU29)
Comments Part of: Allen Cell Collection.
Population: Japanese.
Characteristics: Using CRISPR/Cas9 FBL has been endogenously tagged in one allele at the C-terminus with mEGFP, NPM1 has been endogenously tagged in one allele at the C-terminus with mTagRFP-T and a dCas9-TagBFP-KRAB construct is expressed from a CAGGS promoter edited into the 2nd intron of the CLYBL safe harbor.
Transfected with: UniProtKB; P42212; GFP (with p.Tyr66His and p.Tyr145Phe = BFP).
Transfected with: UniProtKB; H3JQU7; Entacmaea quadricolor red fluorescent protein eqFP578 (mutated to obtain mTagRFP-T).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (deactivated version with KRAB domain; dCas9-KRAB).
Derived from sampling site: Leg; skin. Cell type=Fibroblast.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Web pages https://www.allencell.org/cell-catalog.html
Cell line collections Coriell; AICS-0089-061
Cell line databases/resources hPSCreg; UCSFi001-A-39
Biological sample resources BioSamples; SAMEA7655481
Encyclopedic resources Wikidata; Q98135041
Entry history
Entry creation12-Mar-2020
Last entry update21-Mar-2023
Version number7