ID   WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61
AC   CVCL_YU29
SY   AICS-0089-061; AICSi002-A-39; UCSFi001-A-39
DR   BioSamples; SAMEA7655481
DR   Coriell; AICS-0089-061
DR   hPSCreg; UCSFi001-A-39
DR   Wikidata; Q98135041
WW   Info; Allen Institute; -; https://www.allencell.org/cell-catalog.html
CC   Part of: Allen Cell Collection.
CC   Population: Japanese.
CC   Characteristics: Using CRISPR/Cas9 FBL has been endogenously tagged in one allele at the C-terminus with mEGFP, NPM1 has been endogenously tagged in one allele at the C-terminus with mTagRFP-T and a dCas9-TagBFP-KRAB construct is expressed from a CAGGS promoter edited into the 2nd intron of the CLYBL safe harbor.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; QKFJN; mEGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; LF3LJ; TagRFP-T (Note=Red fluorescent protein derived from eqFP578).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_Y803 ! GM25256
SX   Male
AG   30Y
CA   Induced pluripotent stem cell
DT   Created: 12-03-20; Last updated: 10-04-25; Version: 12
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