Cellosaurus 46C-Rybp KO (CVCL_YJ62)
| Cell line name | 46C-Rybp KO |
|---|---|
| Synonyms | 46C-RYBP KO; GIBHe001-A-1 |
| Accession | CVCL_YJ62 |
| Resource Identification Initiative | To cite this cell line use: 46C-Rybp KO (RRID:CVCL_YJ62) |
| Comments | Characteristics: Using CRISPR/Cas9 the last two exons of Rybp gene were replaced with a PGKp-PuroR construct (PubMed=31794887). Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line). Knockout cell: Method=CRISPR/Cas9; MGI; MGI:1929059; Rybp. Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Caution: Named GIBHe001-A-1 which according to ESC nomenclature rules would imply being a derivative of the GIBHe001-A human ESC cell line, but this is not the case. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola. |
| Hierarchy | Parent: CVCL_Y482 (46C) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=31794887; DOI=10.1016/j.scr.2019.101638 |
| Cross-references | |
| Cell line databases/resources | SKIP; SKIP005593 |
| Encyclopedic resources | Wikidata; Q93307125 |
| Entry history | |
| Entry creation | 19-Dec-2019 |
| Last entry update | 14-Aug-2025 |
| Version number | 9 |