ID   46C-RYBP KO
AC   CVCL_YJ62
SY   GIBHe001-A-1
DR   SKIP; SKIP005593
DR   Wikidata; Q93307125
RX   PubMed=31794887;
CC   Characteristics: Using CRISPR/Cas9 the last two exons of Rybp gene were replaced with PGK-Puro by homologous recombination.
CC   Characteristics: Expresses GFP under the control of the Sox1 promoter.
CC   Knockout cell: Method=CRISPR/Cas9; MGI; MGI:1929059; Rybp.
CC   Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Caution: Named GIBHe001-A-1 which according to ESC nomenclature rules would imply being a derivative of the GIBHe001-A human ESC cell line, but this is not the case.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_9108 ! ES-E14TG2a
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 19-12-19; Last updated: 29-06-23; Version: 6
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RX   PubMed=31794887; DOI=10.1016/j.scr.2019.101638;
RA   Liu Z.-C., Yao M.-Z., Yao H.-J., Hu G.-C., Qin B.-M.;
RT   "Generation of Rybp homozygous knockout murine ES cell line
RT   GIBHe001-A-1 by using CRISPR/Cas9 technology.";
RL   Stem Cell Res. 41:101638-101638(2019).
//