ID   46C-Rybp KO
AC   CVCL_YJ62
SY   46C-RYBP KO; GIBHe001-A-1
DR   SKIP; SKIP005593
DR   Wikidata; Q93307125
RX   PubMed=31794887;
CC   Characteristics: Using CRISPR/Cas9 the last two exons of Rybp gene were replaced with a PGKp-PuroR construct (PubMed=31794887).
CC   Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; MGI; MGI:1929059; Rybp.
CC   Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Caution: Named GIBHe001-A-1 which according to ESC nomenclature rules would imply being a derivative of the GIBHe001-A human ESC cell line, but this is not the case.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_Y482 ! 46C
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 19-12-19; Last updated: 19-12-24; Version: 8
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RX   PubMed=31794887; DOI=10.1016/j.scr.2019.101638;
RA   Liu Z.-C., Yao M.-Z., Yao H.-J., Hu G.-C., Qin B.-M.;
RT   "Generation of Rybp homozygous knockout murine ES cell line
RT   GIBHe001-A-1 by using CRISPR/Cas9 technology.";
RL   Stem Cell Res. 41:101638-101638(2019).
//