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Cellosaurus U2OS-tetR-PAmCherry1-KRAS G12D (CVCL_XZ89)

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Cell line name U2OS-tetR-PAmCherry1-KRAS G12D
Accession CVCL_XZ89
Resource Identification Initiative To cite this cell line use: U2OS-tetR-PAmCherry1-KRAS G12D (RRID:CVCL_XZ89)
Comments Population: Caucasian.
Characteristics: The PAmCherry1 fluorescent protein is fused to the N-terminal of KRAS with p.Gly12Asp (PubMed=31674905).
Genetic integration: Method=Transduction; Gene=FPbase; OMODZ; PAmCherry1 (Note=Photoactivatable red fluorescent protein derived from mCherry).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:6407; KRAS (Note=With p.Gly12Asp (c.35G>A)).
Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_XZ88 (U2OS-tetR)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=31674905; DOI=10.7554/eLife.46393; PMCID=PMC7060040
Yerim Lee, Carey Phelps, Tao Huang, Barmak Mostofian, Lei Wu, Ying Zhang, Kai Tao, Young-Hwan Chang, Philip J.S. Stork, Joe W. Gray, Daniel M. Zuckerman, Xiao-Lin Nan;
High-throughput, single-particle tracking reveals nested membrane domains that dictate KRas(G12D) diffusion and trafficking.
eLife 8:e46393.1-e46393.23(2019)

Cross-references
Encyclopedic resources Wikidata; Q98133790
Entry history
Entry creation19-Dec-2019
Last entry update10-Apr-2025
Version number11