ID   U2OS-tetR-PAmCherry1-KRAS G12D
AC   CVCL_XZ89
DR   Wikidata; Q98133790
RX   PubMed=31674905;
CC   Population: Caucasian.
CC   Characteristics: The PAmCherry1 fluorescent protein is fused to the N-terminal of KRAS with p.Gly12Asp (PubMed=31674905).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=FPbase; OMODZ; PAmCherry1 (Note=Photoactivatable red fluorescent protein derived from mCherry).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:6407; KRAS (Note=With p.Gly12Asp (c.35G>A)).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_XZ88 ! U2OS-tetR
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 19-12-19; Last updated: 10-04-25; Version: 11
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RX   PubMed=31674905; DOI=10.7554/eLife.46393; PMCID=PMC7060040;
RA   Lee Y., Phelps C., Huang T., Mostofian B., Wu L., Zhang Y., Tao K.,
RA   Chang Y.-H., Stork P.J.S., Gray J.W., Zuckerman D.M., Nan X.-L.;
RT   "High-throughput, single-particle tracking reveals nested membrane
RT   domains that dictate KRas(G12D) diffusion and trafficking.";
RL   eLife 8:e46393.1-e46393.23(2019).
//